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A Multiplex PCR Assay for Different-iation of Streptococcus parauberis Serotypes

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タイトル: A Multiplex PCR Assay for Different-iation of Streptococcus parauberis Serotypes
著者: Tu, Chuandeng / Suga, Koushirou / Kanai, Kinya
発行日: 2015年12月29日
出版者: 日本魚病学会 / Japanese Society of Fish Pathology
引用: Fish Pathology, 50(4), pp.213-215; 2015
抄録: A multiplex PCR assay for differentiation of Streptococcus parauberis serotypes was developed. The three primer pairs for subserotypes Ia and Ib/Ic and serotype II were designed from the serotype-specific sequences of the wzy gene in the loci for capsular polysaccharide biosynthesis. All of 188 S. parauberis isolates from Japanese flounder showed positive reaction with the expected size of PCR product for each serotype, which was consistent with the results of agglutination test using rabbit antisera. The nontypeable isolates, which are not differentiated by agglutination test, could be identified as subzero type Ib/Ic or serotype II. Other streptococci including S. parauberis derived from cow and major bacterial fish pathogens showed negative reaction.
キーワード: Streptococcus parauberis / multiplex PCR / serotyping / streptococcosis
URI: http://hdl.handle.net/10069/36676
ISSN: 0388788X
DOI: 10.3147/jsfp.50.213
権利: © 2015 The Japanese Society of Fish Pathology
資料タイプ: Journal Article
原稿種類: publisher
出現コレクション:080 学術雑誌論文

引用URI : http://hdl.handle.net/10069/36676



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