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A Multiplex PCR Assay for Different-iation of Streptococcus parauberis Serotypes

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Title: A Multiplex PCR Assay for Different-iation of Streptococcus parauberis Serotypes
Authors: Tu, Chuandeng / Suga, Koushirou / Kanai, Kinya
Issue Date: 29-Dec-2015
Publisher: 日本魚病学会 / Japanese Society of Fish Pathology
Citation: Fish Pathology, 50(4), pp.213-215; 2015
Abstract: A multiplex PCR assay for differentiation of Streptococcus parauberis serotypes was developed. The three primer pairs for subserotypes Ia and Ib/Ic and serotype II were designed from the serotype-specific sequences of the wzy gene in the loci for capsular polysaccharide biosynthesis. All of 188 S. parauberis isolates from Japanese flounder showed positive reaction with the expected size of PCR product for each serotype, which was consistent with the results of agglutination test using rabbit antisera. The nontypeable isolates, which are not differentiated by agglutination test, could be identified as subzero type Ib/Ic or serotype II. Other streptococci including S. parauberis derived from cow and major bacterial fish pathogens showed negative reaction.
Keywords: Streptococcus parauberis / multiplex PCR / serotyping / streptococcosis
URI: http://hdl.handle.net/10069/36676
ISSN: 0388788X
DOI: 10.3147/jsfp.50.213
Rights: © 2015 The Japanese Society of Fish Pathology
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/36676

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