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Haploinsufficiency of interferon regulatory factor 4 strongly protects against autoimmune diabetes in NOD mice


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Title: Haploinsufficiency of interferon regulatory factor 4 strongly protects against autoimmune diabetes in NOD mice
Other Titles: IRF4遺伝子ヘテロ欠損NODマウスは、自己免疫性糖尿病が高度に抑制される
Authors: 赤澤, 諭
Authors (alternative): Akazawa, Satoru
Issue Date: 3-Feb-2016
Publisher: Springer-Verlag
Citation: Nagasaki University (長崎大学), 博士(医学) (2016-02-03)
Abstract: Aims/hypothesis: Interferon regulatory factor (IRF)4 plays a critical role in lymphoid development and the regulation of immune responses. Genetic deletion of IRF4 has been shown to suppress autoimmune disease in several mouse models, but its role in autoimmune diabetes in NOD mice remains unknown. Methods: To address the role of IRF4 in the pathogenesis of autoimmune diabetes in NOD mice, we generated IRF4-knockout NOD mice and investigated the impact of the genetic deletion of IRF4 on diabetes, insulitis and insulin autoantibody; the effector function of T cells in vivo and in vitro; and the proportion of dendritic cell subsets. Results: Heterozygous IRF4-deficient NOD mice maintained the number and phenotype of T cells at levels similar to NOD mice. However, diabetes and autoantibody production were completely suppressed in both heterozygous and homozygous IRF4-deficient NOD mice. The level of insulitis was strongly suppressed in both heterozygous and homozygous IRF4-deficient mice, with minimal insulitis observed in heterozygous mice. An adoptive transfer study revealed that IRF4 deficiency conferred disease resistance in a gene-dose-dependent manner in recipient NOD/severe combined immunodeficiency mice. Furthermore, the proportion of migratory dendritic cells in lymph nodes was reduced in heterozygous and homozygous IRF4-deficient NOD mice in an IRF4 dose-dependent manner. These results suggest that the levels of IRF4 in T cells and dendritic cells are important for the pathogenesis of diabetes in NOD mice. Conclusions/interpretation: Haploinsufficiency of IRF4 halted disease development in NOD mice. Our findings suggest that an IRF4-targeted strategy might be useful for modulating autoimmunity in type 1 diabetes.
Description: 長崎大学学位論文 学位記番号:博(医歯薬)甲第821号 学位授与年月日:平成28年2月3日 / Author: Satoru Akazawa, Masakazu Kobayashi, Genpei Kuriya, Ichiro Horie, Liping Yu, Hironori Yamasaki, Minoru Okita, Yuji Nagayama, Toshifumi Matsuyama, Masoud Akbari, Katsuyuki Yui, Atsushi Kawakami, Norio Abiru / Citation: Diabetologia, 58(11), pp.2606-2614; 2015
Keywords: Autoimmunity / Dendritic cells / Interferon regulatory factor 4 / NOD mice / T cells / Type 1 diabetes
URI: http://hdl.handle.net/10069/36939
ISSN: 0012186X
DOI: 10.1007/s00125-015-3724-3
Relational Links: http://hdl.handle.net/10069/36272
Rights: (C) Springer-Verlag Berlin Heidelberg 2015. / The final publication is available at link.springer.com.
Type: Thesis or Dissertation
Text Version: ETD
Appears in Collections:dissertation

Citable URI : http://hdl.handle.net/10069/36939

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