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Efficacy of freeze-dried platelet-rich plasma in bone engineering


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Title: Efficacy of freeze-dried platelet-rich plasma in bone engineering
Other Titles: フリーズドライ保存した多血小板血漿は骨再生に有用である
Authors: 中谷, 佑哉
Authors (alternative): Nakatani, Yuya
Issue Date: 1-Feb-2017
Publisher: Elsevier
Citation: Nagasaki University (長崎大学), 博士(歯学) (2017-02-01)
Abstract: Objective: Platelet-rich plasma (PRP) is typically isolated and applied immediately after preparation, making it both a time- and labor-intensive addition to the operative procedure. Thus, it would be convenient if PRP could be preserved. We evaluated the efficacy of freeze-dried PRP (FD-PRP), as compared with freshly isolated PRP (f-PRP) for bone engineering. Design: FD-PRP was prepared by lyophilization of f-PRP and was subsequently preserved at −20 °C for one month. It was then rehydrated with an equal or 1/3 amount of distilled water (×1FD-PRP, ×3FD-PRP, respectively), and we assessed its gelation properties and the release of growth factors (PDGF-BB, TGF-β1, and VEGF). We also examined the bone forming ability with onlay-grafting on mice calvaria using β-TCP granules as a scaffold. Results: FD-PRP showed comparable gelation as f-PRP. In terms of growth factor release, × 1FD-PRP released identical concentrations of PDGF-BB and TGF-β1 to f-PRP, while ×3FD-PRP released approximately 3-fold concentrations when compared with f-PRP. In vivo, ×1FD-PRP promoted identical levels of the bone formation as f-PRP, and ×3FD-PRP induced more abundant bone formation. Conclusions: These results suggest that f-PRP can be stored without functional loss by freeze-drying and the concentration of PRP may improve its efficacy in bone engineering.
Description: 長崎大学学位論文 学位記番号:博(医歯薬)甲第913号 学位授与年月日:平成29年2月1日 / Author: Yuya Nakatani, Hideki Agata, Yoshinori Sumita, Takamitsu Koga, Izumi Asahina / Citation: Archives of Oral Biology, 73, pp.172-178; 2017
Keywords: Platelet-rich plasma / Freeze-Dry / Long storage / Super-concentrated platelets / Bone regeneration
URI: http://hdl.handle.net/10069/37042
ISSN: 00039969
DOI: 10.1016/j.archoralbio.2016.10.006
Rights: © 2016 Elsevier Ltd. All rights reserved.
Type: Thesis or Dissertation
Text Version: ETD
Appears in Collections:dissertation

Citable URI : http://hdl.handle.net/10069/37042

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