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Chondroitin sulfate prevents peritoneal fibrosis in mice by suppressing NF-κB activation


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Title: Chondroitin sulfate prevents peritoneal fibrosis in mice by suppressing NF-κB activation
Other Titles: コンドロイチン硫酸はNF-κB活性化を抑制することでマウスの腹膜線維化を抑制する
Authors: 阿部, 伸一
Authors (alternative): Abe, Shinichi
Issue Date: 1-Jun-2016
Publisher: Springer Japan
Citation: Nagasaki University (長崎大学), 博士(医学) (2016-06-01)
Abstract: Long-term peritoneal dialysis causes peritoneal fibrosis, and previous reports suggest that inflammation plays a critical role in peritoneal fibrosis. Chondroitin sulfate (CS) suppresses the inflammatory response by preventing activation of nuclear factor (NF)-κB. We examined the effect of CS on the peritoneal fibrosis induced by chlorhexidine gluconate (CG) in mice. CS or water was administered daily. We divided mice into four groups: administered vehicle and water (control); administered vehicle and CS (CS); administered CG and water (CG); and administered CG and CS (CG+CS). Morphologic changes were assessed by Masson’s trichrome staining. Inflammation- and fibrosis-associated factors were assessed by immunohistochemistry. Activation of NF-κB was examined by southwestern histochemistry. CS administration suppressed the progression of submesothelial thickening. The numbers of inflammation- and fibrosis-associated factors -positive cells were significantly decreased in the CG+CS group, compared to the CG group. Based on SWH, the CG+CS group contained significantly fewer NF-κB-activated cells than the CG group. Our results indicate that CS suppresses peritoneal fibrosis via suppression of NF-κB activation. These results suggest that CS has therapeutic potential for peritoneal fibrosis.
Description: 長崎大学学位論文 学位記番号:博(医歯薬)甲第874号 学位授与年月日:平成28年6月1日 / Author: Shinichi Abe, Yoko Obata, Satoru Oka, Takehiko Koji, Tomoya Nishino, Koichi Izumikawa / Citation: Medical Molecular Morphology, 49(3), pp.144-153; 2016
Keywords: Chondroitin sulfate / Peritoneal fibrosis / NF-κB / Mitogen-activated protein kinase / Chlorhexidine gluconate
URI: http://hdl.handle.net/10069/37054
ISSN: 18601480
DOI: 10.1007/s00795-016-0133-8
Relational Links: http://hdl.handle.net/10069/36594
Rights: (C) The Japanese Society for Clinical Molecular Morphology 2016 / The original publication is available at www.springerlink.com
Type: Thesis or Dissertation
Text Version: ETD
Appears in Collections:dissertation

Citable URI : http://hdl.handle.net/10069/37054

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