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Diversity of multinucleated giant cells by microstructures of hydroxyapatite and plasma components in extraskeletal implantation model


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Title: Diversity of multinucleated giant cells by microstructures of hydroxyapatite and plasma components in extraskeletal implantation model
Other Titles: 皮下移植実験系においてハイドロキシアパタイトの微細構造及び血漿成分の影響を受けて誘導される多様な多核巨細胞
Authors: 森下, 廣太
Authors (alternative): Morishita, Kota
Issue Date: 21-Mar-2017
Publisher: Elsevier Ltd.
Citation: Nagasaki University (長崎大学), 博士(歯学) (2017-03-21)
Abstract: Foreign body giant cells (FBGCs) and osteoclasts are multinucleated giant cells (MNGCs), both of which are formed by the fusion of macrophage-derived mononuclear cells. Osteoclasts are distinct from FBGCs due to their bone resorption ability; however, not only morphological, but also functional similarities may exist between these cells. The characterization and diversity of FBGCs that appear in an in vivo foreign body reaction currently remain incomplete. In the present study, we investigated an in vivo foreign body reaction using an extraskeletal implantation model of hydroxyapatite (HA) with different microstructures. The implantation of HA granules in rat subcutaneous tissue induced a foreign body reaction that was accompanied by various MNGCs. HA granules composed of rod-shaped particles predominantly induced cathepsin K (CTSK)-positive FBGCs, whereas HA granules composed of globular-shaped particles predominantly induced CTSK-negative FBGCs. Plasma, which was used as the binder of ceramic granules, stimulated the induction of CTSK-positive FBGCs more strongly than purified fibrin. Furthermore, the implantation of HA composed of rod-shaped particles with plasma induced tartrate-resistant acid phosphatase (TRAP)-positive MNGCs in contrast to HA composed of globular-shaped particles with purified fibrin, which predominantly induced CTSK-negative and TRAP-negative typical FBGCs. These results suggest that CTSK-positive, TRAP-positive, and CTSK- and TRAP-negative MNGCs are induced in this subcutaneous implantation model in a manner that is dependent on the microstructure of HA and presence or absence of plasma.
Description: 長崎大学学位論文 学位記番号:博(医歯薬)甲第923号 学位授与年月日:平成29年3月21日 / Author: Kota Morishita, Eri Tatsukawa, Yasuaki Shibata, Fumio Suehiro, Masanobu Kamitakahara, Taishi Yokoi, Koji Ioku, Masahiro Umeda, Masahiro Nishimura, Tohru Ikeda / Citation: Acta Biomaterialia, 39, pp.180-191; 2016
Keywords: Foreign body giant cell / Osteoclast / Hydroxyapatite / Microstructure / Cathepsin K
URI: http://hdl.handle.net/10069/37099
ISSN: 17427061
DOI: 10.1016/j.actbio.2016.05.002
Rights: © 2016 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
Type: Thesis or Dissertation
Text Version: ETD
Appears in Collections:dissertation

Citable URI : http://hdl.handle.net/10069/37099

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