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Protective effect of porphyran isolated from discolored nori (Porphyra yezoensis) on lipopolysaccharide-induced endotoxin shock in mice

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タイトル: Protective effect of porphyran isolated from discolored nori (Porphyra yezoensis) on lipopolysaccharide-induced endotoxin shock in mice
著者: Nishiguchi, Tomoki / Cho, Kichul / Isaka, Shogo / Ueno, Mikinori / Jin, Jun-O / Yamaguchi, Kenichi / Kim, Daekyung / Oda, Tatsuya
発行日: 2016年12月
出版者: Elsevier B.V.
引用: International Journal of Biological Macromolecules, 93(Part A), pp.1273-1278; 2016
抄録: Porphyran, a sulfated polysaccharide, isolated from discolored nori (Porphyra yezoensis) (dc-porphyran) and one fraction (F1) purified from dc-porphyran by DEAE-chromatography showed the protective effects on LPS-induced endotoxin shock in mice. Intraperitoneal (i.p.) treatment with dc-porphyran or F1 (100 mg/kg) 60 min prior to i.p. injection of LPS (30 mg/kg) completely protected mice from LPS lethality. At 10 mg/kg concentration, F1 demonstrated more protection than dc-porphyran. Intravenous (i.v.) challenge of LPS, even at 20 mg/kg, was more lethal than i.p. administration; i.v. injection of F1 (100 mg/kg) with LPS significantly improved the survival rate. However, i.v. dc-porphyran (100 mg/kg) produced an even lower survival rate than that of LPS alone. We examined pro-inflammatory mediators such as NO and TNF-α in serum. F1 significantly reduced the levels of these markers. Additionally, F1 significantly decreased the malondialdehyde level in the liver, a marker of oxidative stress, while dc-porphyran had almost no effect. Furthermore, F1 significantly decreased the production of TNF-α and NO in peritoneal exudate cells harvested from LPS-challenged mice, while dc-porphyran treatment showed a lesser decrease. Our results suggest that porphyran isolated from discolored nori, especially F1, is capable of suppressing LPS-induced endotoxin shock in vivo.
キーワード: Porphyra yezoensis / porphyran / LPS / endotoxin shock / anti-inflammatory activity
URI: http://hdl.handle.net/10069/37292
ISSN: 01418130
DOI: 10.1016/j.ijbiomac.2016.09.091
権利: © 2016 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
資料タイプ: Journal Article
原稿種類: publisher
出現コレクション:080 学術雑誌論文

引用URI : http://hdl.handle.net/10069/37292



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