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Anti-MUC1 Aptamer/Negatively Charged Amino Acid Dendrimer Conjugates for Targeted Delivery to Human Lung Adenocarcinoma A549 Cells


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Title: Anti-MUC1 Aptamer/Negatively Charged Amino Acid Dendrimer Conjugates for Targeted Delivery to Human Lung Adenocarcinoma A549 Cells
Authors: Masuda, Marie / Kawakami, Shigeru / Wijagkanalan, Wassana / Suga, Tadaharu / Fuchigami, Yuki / Yamashita, Fumiyoshi / Hashida, Mitsuru
Issue Date: 1-Oct-2016
Publisher: 日本薬学会 / Pharmaceutical Society of Japan
Citation: Biological & Pharmaceutical Bulletin, 39(10), pp.1734-1738; 2016
Abstract: We previously developed a negatively charged amino acid dendrimer to address the safety concerns associated with the constituent unit of these systems, which resulted in the formation of a sixth-generation glutamic acid-modified dendritic poly(L-lysine) system (KG6E). The aim of this study was to develop a nanocarrier for targeted drug delivery into cancer cells. In this study, we have synthesized a conjugate material consisting of anti-mucin 1 (MUC1) aptamer (anti-MUC1 apt) and KG6E (anti-MUC1 apt/KG6E) for targeted drug delivery to human lung adenocarcinoma A549 cells, which express high levels of the MUC1. The anti-MUC1 apt/KG6E was efficiently internalized by the A549 cells and subsequently transported to the endosomal and lysosomal compartments. In contrast, the cellular association of the sequence scrambled aptamer/KG6E conjugate (scrambled apt/KG6E) was much lower than that of the anti-MUC1 apt/KG6E in A549 cells. These results suggest that our newly developed anti-MUC1 apt/KG6E can be internalized in A549 cells via a MUC1 recognition pathway.
Keywords: Aptamer / Dendrimer / Drug delivery / Mucin 1 (MUC1) / Targeting
URI: http://hdl.handle.net/10069/37298
ISSN: 09186158
DOI: 10.1248/bpb.b16-00508
Rights: © 2016 The Pharmaceutical Society of Japan.
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/37298

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