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Evaluation for Peritoneal Injury at an Early Stage Using Dual Macromolecular Markers


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Title: Evaluation for Peritoneal Injury at an Early Stage Using Dual Macromolecular Markers
Authors: Hirata, Haruna / Fumoto, Shintaro / Miyamoto, Hirotaka / Nakashima, Mikiro / Nakayama, Morio / Nishida, Koyo
Issue Date: 1-Oct-2016
Publisher: 日本薬学会 / Pharmaceutical Society of Japan
Citation: Biological & Pharmaceutical Bulletin, 39(10), pp.1581-1587; 2016
Abstract: Long-term peritoneal dialysis (PD) frequently produces morphological and functional changes of the peritoneum, making continuation of PD difficult. Therefore, it is necessary to evaluate peritoneal injury at an early stage and develop appropriate therapies. The aims of the present study were to evaluate peritoneal injury at an early stage and assess a drug for prevention of peritoneal injury using our previously developed novel evaluation method. Peritoneal injury was induced in model animals by intraperitoneal injection of methylglyoxal (MGO) for 1 to 5 consecutive days or chlorhexidine digluconate (CG) for 1 to 14 consecutive days. Tetramethylrhodamine-dextran (RD)-10 and fluorescein isothiocyanate-dextran (FD)-2000 were then injected into the peritoneal cavity and recovered after 120 min to evaluate peritoneal injury. The ratio of the concentration of RD-10 to FD-2000 (RD-10/FD-2000 ratio) significantly decreased in animals that had been treated with MGO or CG for 1 d. Moreover, the RD-10/FD-2000 ratio significantly increased in CG- and thalidomide-treated animals. The RD-10/FD-2000 ratio can be used to evaluate peritoneal injury at an early stage and assess the drug efficacy of thalidomide for prevention of peritoneal injury. This study will contribute to the development of therapeutic treatments for peritoneal injury.
Keywords: Early peritoneal injury / Hyperpermeability / Macromolecular marker / Peritoneal dialysis
URI: http://hdl.handle.net/10069/37300
ISSN: 09186158
DOI: 10.1248/bpb.b15-01042
Rights: © 2016 The Pharmaceutical Society of Japan.
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/37300

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