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Dose-dependency and reversibility of radiation-induced injury in cardiac explant-derived cells of mice


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タイトル: Dose-dependency and reversibility of radiation-induced injury in cardiac explant-derived cells of mice
著者: Luo, Lan / Yan, Chen / Urata, Yoshishige / Hasan, Al Shaimaa / Goto, Shinji / Guo, Chang-Ying / Zhang, Shouhua / Li, Tao-Sheng
発行日: 2017年 1月18日
出版者: Macmillan Publishers Limited
引用: Scientific Reports, 7, 40959; 2017
抄録: We evaluated the dose-dependency and reversibility of radiation-induced injury in cardiac explant-derived cells (CDCs), a mixed cell population grown from heart tissues. Adult C57BL/6 mice were exposed to 0, 10, 50 and 250 mGy γ 3-rays for 7 days and atrial tissues were collected for experiments 24 hours after last exposure. The number of CDCs was significantly decreased by daily exposure to over 250 mGy. Interestingly, daily exposure to over 50 mGy significantly decreased the c-kit expression and telomerase activity, increased 53BP1 foci in the nuclei of CDCs. However, CD90 expression and growth factors production in CDCs were not significantly changed even after daily exposure to 250 mGy. We further evaluated the reversibility of radiation-induced injury in CDCs at 1 week and 3 weeks after a single exposure to 3 Gy γ 3-rays. The number and growth factors production of CDCs were soon recovered at 1 week. However, the increased expression of CD90 were retained at 1 week, but recovered at 3 weeks. Moreover, the decreased expression of c-kit, impaired telomerase activity, and increased 53BP1 foci were poorly recovered even at 3 weeks. These data may help us to find the most sensitive and reliable bio-parameter(s) for evaluating radiation-induced injury in CDCs.
URI: http://hdl.handle.net/10069/37426
DOI: 10.1038/srep40959
権利: © The Author(s) 2017. / This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
資料タイプ: Journal Article
原稿種類: publisher
出現コレクション:115 学術雑誌論文

引用URI : http://hdl.handle.net/10069/37426

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