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Dominant prevalence of Porphyromonas gingivalis fimA types I and IV in healthy Japanese children


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タイトル: Dominant prevalence of Porphyromonas gingivalis fimA types I and IV in healthy Japanese children
著者: Shimoyama, Yu / Ohara-Nemoto, Yuko / Kimura, Misumi / Nemoto, Takayuki K. / Tanaka, Mitsuro / Kimura, Shigenobu
発行日: 2017年 9月
出版者: Association for Dental Sciences of the Republic of China
引用: Journal of Dental Sciences, 12, 213-219; 2017
抄録: Background/purpose: Porphyromonas gingivalis is a major causative agent of chronic periodontitis, whilst circumstances for acquisition of the bacterium remain to be elucidated. To examine prevalence of the bacterium harboring distinct fimA types in dental plaque of children, we established PCR procedures that are applicable for specimens with limited amounts. By this method, all six fimA types including type I and Ib were directly identified, and prevalence of fimA types and their frequency of guardian-child transmission in Japanese children were assessed. Materials and methods: Genomic DNA was purified from dental plaque specimens of 132 periodontally healthy children (2-12 years old, 4.8 ± 0.2 years) and 19 mothers of resultant P. gingivalis-positive child subjects. PCR-based fimA genotyping was performed, and untypeable strains in the first PCR analysis were determined by a nested PCR. Results: P. gingivalis was found in 15.2% of the subjects (2-10 years old, 5.1 ± 0.6 years), and the most prevalent types were I and IV (37.0% each), followed by Ib and III (11.1% each), and then II (7.4%). Seven (35.0%) of the 20 P. gingivalis-positive subjects had combined colonization of type I with other fimA types. In most cases, bacterial prevalence and fimA types in the children were distinct from those of their mothers, indicating that its maternal transmission was not significant. Conclusion: These results suggest that colonization of non-disease-associated fimA types I and IV P. gingivalis to the oral cavity initiates from early childhood without showing any periodontal inflammation.
キーワード: Child / Colonization / Dental plaque / fimA / Genotyping / Porphyromonas gingivalis
URI: http://hdl.handle.net/10069/37552
ISSN: 19917902
DOI: 10.1016/j.jds.2017.03.006
権利: © 2017 Association for Dental Sciences of the Republic of China. Publishing services by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
資料タイプ: Journal Article
原稿種類: publisher
出現コレクション:040 学術雑誌論文

引用URI : http://hdl.handle.net/10069/37552

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