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Development of an Evaluation Method for Hydroxyl Radical Scavenging Activities Using Sequential Injection Analysis with Chemiluminescence Detection


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Title: Development of an Evaluation Method for Hydroxyl Radical Scavenging Activities Using Sequential Injection Analysis with Chemiluminescence Detection
Authors: Miyamoto, Aoi / Nakano, Saori / Nagai, Kaishu / Kishikawa, Naoya / Ohyama, Kaname / Aoyama, Takahiko / Matsumoto, Yoshiaki / Kuroda, Naotaka
Issue Date: 10-Jun-2017
Publisher: 日本分析化学会 / Japan Society for Analytical Chemistry
Citation: Analytical Sciences, 33(6), pp.697-701; 2017
Abstract: A method for evaluating hydroxyl radical (·OH) scavenging activities using sequential injection analysis (SIA) with chemiluminescence (CL) detection was developed. In this system, CL was produced by the reaction of luminol with ·OH generated from the Fenton reaction. The scavenging activity was expressed as a diminution rate of the CL due to the scavenging of ·OH by a sample. The SIA system allows the automation of a series of experimental procedures including Fenton's reaction, scavenging of ·OH, and luminol CL reaction. The evaluation of scavenging activities in one sample (n = 3) was completed within 3.0 min. Relative standard deviations (n = 3) of scavenging activity with 700 μM L-ascorbic acid were 2.6% (intraday) and 3.7% (interday). The SIA-CL system was applied to measure ·OH scavenging activities of several antioxidants and pharmaceuticals.
Keywords: Chemiluminescence / Hydroxyl radical / Luminol / Sequential injection analysis
URI: http://hdl.handle.net/10069/37597
ISSN: 09106340
DOI: 10.2116/analsci.33.697
Rights: © 2017 The Japan Society for Analytical Chemistry
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/37597

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