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Macrophage-stimulating activities of newly isolated complex polysaccharides from Parachlorella kessleri strain KNK-A001

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タイトル: Macrophage-stimulating activities of newly isolated complex polysaccharides from Parachlorella kessleri strain KNK-A001
著者: Ueno, Mikinori / Cho, Kichul / Hirata, Narumi / Yamashita, Kenji / Yamaguchi, Kenichi / Kim, Daekyung / Oda, Tatsuya
発行日: 2017年11月
引用: International Journal of Biological Macromolecules, 104, pp.400-406; 2017
抄録: Our previous studies demonstrated that the microalga Parachlorella kessleri (KNK-A001) has immunostimulatory activities, which were observed as an increase in natural killer (NK) cell activity in mice after intraperitoneal injection or as a protective effect on a virus-infected model shrimp after oral administration. In this study, we attempted to gain insight into the constituent substances of KNK-A001 that are responsible for the immunostimulatory activity. First, we obtained five polysaccharide fractions from KNK-A001 by DEAE anion exchange chromatography. Among the fractions, F5 showed the most potent induction of nitric oxide (NO) secretion in RAW264.7 cells, and both mRNA and protein expression levels of inducible NO synthase (iNOS) were increased in F5-treated RAW264.7 cells. A significant increase in the nuclear translocation of the p65 subunit of nuclear factor-kappa B (NF-κB) was observed in F5-treated RAW264.7 cells. F5 also induced the secretion of tumor necrosis factor (TNF)-α in RAW264.7 cells. Analysis using mitogen-activated protein (MAP) kinase inhibitors suggested that c-Jun N-terminal kinase (JNK) and p38 MAP kinase were mainly involved in F5-induced NO and TNF-α productions. The compositional analysis of F5 identified the main constituents as galactose, glucose, galacturonic acid, and mannose. Gel-filtration analysis suggested that molecular mass of F5 was approximately 400 kDa.
キーワード: Macrophages / Nitric oxide / Parachlorella kessleri / Polysaccharides / NF-κB / TNF-α
URI: http://hdl.handle.net/10069/37624
ISSN: 01418130
DOI: 10.1016/j.ijbiomac.2017.06.014
権利: © 2017 Elsevier B.V. All rights reserved. / This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
資料タイプ: Journal Article
原稿種類: author
出現コレクション:080 学術雑誌論文

引用URI : http://hdl.handle.net/10069/37624



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