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Effective intraperitoneal gene transfection system using nanobubbles and ultrasound irradiation


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タイトル: Effective intraperitoneal gene transfection system using nanobubbles and ultrasound irradiation
著者: Nishimura, Koyo / Fumoto, Shintaro / Fuchigami, Yuki / Hagimori, Masayori / Maruyama, Kazuo / Kawakami, Shigeru
発行日: 2017年 4月27日
出版者: Informa UK Limited
引用: Drug Delivery, 24(1), pp.737-744; 2017
抄録: In this study, we demonstrate the low toxicity and highly efficient and spatially improved transfection of plasmid DNA (pDNA) with liposomal nanobubbles (bubble liposomes [BLs]) using ultrasound (US) irradiation in mice. Naked pDNA with BLs was intraperitoneally injected, followed by US irradiation. The injection volume, the duration of US irradiation, and the dose of BLs were optimized. Both BLs and US irradiation were essential to achieve high transgene expression from naked pDNA. We observed transgene expression in the entire peritoneal tissues, including the peritoneal wall, liver, spleen, stomach and small and large intestines. The area of transfection could be controlled with focused US irradiation. There were few changes in the morphology of the peritoneum, the peritoneal function or serum alanine aminotransferase levels, suggesting the safety of BLs with US irradiation. Using a tissue-clearing method, the spatial distribution of transgene expression was evaluated. BLs with US irradiation delivered pDNA to the submesothelial layer in the peritoneal wall, whereas transgene expression was restricted to the surface layer in the liver and stomach. Therefore, BLs with US irradiation could be an effective and safe method of gene transfection to the peritoneum.
キーワード: Bubble liposomes / ultrasound / plasmid DNA / intraperitoneal injection / gene therapy
URI: http://hdl.handle.net/10069/37720
ISSN: 10717544
DOI: 10.1080/10717544.2017.1319433
権利: ©2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. / This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/ 4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
資料タイプ: Journal Article
原稿種類: publisher
出現コレクション:050 学術雑誌論文

引用URI : http://hdl.handle.net/10069/37720

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