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Ultrasensitive determination of pyrroloquinoline quinone in human plasma by HPLC with chemiluminescence detection using the redox cycle of quinone


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タイトル: Ultrasensitive determination of pyrroloquinoline quinone in human plasma by HPLC with chemiluminescence detection using the redox cycle of quinone
著者: Fukuda, Mizuho / El-Maghrabey, Mahmoud H. / Kishikawa, Naoya / Ikemoto, Kazuto / Kuroda, Naotaka
発行日: 2017年10月25日
出版者: Elsevier B.V.
引用: Journal of Pharmaceutical and Biomedical Analysis, 145, pp.814-820; 2017
抄録: A fast, accurate, and ultrasensitive high-performance liquid chromatography method with chemiluminescence detection (HPLC-CL) was optimized and validated for the determination of pyrroloquinoline quinone (PQQ) concentration in human plasma following solid-phase extraction (SPE). This method is based on the redox cycle of the reaction between PQQ and dithiothreitol, which generates reactive oxygen species that can be detected using luminol as a CL probe. The isocratic HPLC system comprised an ODS column and 4.0 mM tetra-n-butylammonium bromide in Tris-HNO3 buffer (pH 8.8; 50 mM)-acetonitrile (7:3, v/v) as mobile phase. A novel, rapid, and simple SPE method was also developed providing excellent %recovery (≥95.2%) for PQQ from human plasma samples. The proposed method was linear over the range of 4.0–400 nmol/L plasma of PQQ with a lower detection limit (S/N=3) of 1.08 nmol/L plasma (0.27 nM). The method was successfully implemented to determine PQQ concentration in the plasma of healthy individuals after administration of PQQ supplements.
キーワード: Pyrroloquinoline quinone / HPLC / Chemiluminescence / Redox cycle / Human plasma
URI: http://hdl.handle.net/10069/37761
ISSN: 07317085
DOI: 10.1016/j.jpba.2017.08.008
権利: © 2017 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
資料タイプ: Journal Article
原稿種類: author
出現コレクション:050 学術雑誌論文

引用URI : http://hdl.handle.net/10069/37761

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