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Autologous adipose-derived stem cell sheets enhance the strength of intestinal anastomosis

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タイトル: Autologous adipose-derived stem cell sheets enhance the strength of intestinal anastomosis
その他のタイトル: 自家脂肪由来幹細胞シートは消化管縫合部の強度を増強する
著者: 丸屋, 安広
著者(別表記) : Maruya, Yasuhiro
発行日: 2017年 9月 6日
出版者: The Japanese Society for Regenerative Medicine
引用: Nagasaki University (長崎大学), 博士(医学) (2017-09-06)
抄録: Objective: Adipose-derived stem cells (ASCs) are capable of multiple differentiation pathways, imparting immunomodulatory effects, and secreting factors that are important for wound healing. These characteristics can be exploited to decrease the incidence of anastomotic leakage. Methods: In order to delay local wound healing at the anastomotic site, we induced ischemia in a portion of porcine small intestine by ligating vessels. Then, we injected mitomycin C into the serosa of the small intestine above the ligated vessels. Anastomotic sites were created by 2 cm incisions made in the opposite mesenteric area. ASCs were isolated from the porcine subcutaneous fat tissues and expanded under culture conditions. ASCs were trypsinized and seeded on temperature-responsive dishes and cultured to form confluent sheets. Three ASC sheets were transplanted onto the serous membrane after suturing. The extent of anastomotic wound healing was evaluated by bursting pressure, hydroxyproline content, and mRNA expression of collagen-1 alpha1 and collagen-3 alpha1. Results: We found that transplantation of ASC sheets increased anastomotic site bursting pressure. Additionally, transplantation of ASC sheets increased the hydroxyproline content of the anastomoses. Furthermore, transplantation of ASC sheets increased mRNA expression of collagen-1 alpha1 and collagen-3 alpha1. Conclusions: Our findings showed that transplantation of autologous ASC sheets enhanced collagen synthesis and anastomotic strength. Further studies are necessary to identify substances that, in combination with ASC sheets, might enhance collagen synthesis and healing in sites of anastomosis.
記述: 長崎大学学位論文 学位記番号:博(医歯薬)甲第989号 学位授与年月日:平成29年9月6日 / Author: Yasuhiro Maruya, Nobuo Kanai, Shinichiro Kobayashi, Kurodo Koshino, Teruo Okano, Susumu Eguchi, Masayuki Yamato / Citation: Regenerative Therapy, 7, pp.24-33; 2017
キーワード: Adipose-derived stem cells / Prevent anastomotic leakage
URI: http://hdl.handle.net/10069/37828
ISSN: 23523204
DOI: 10.1016/j.reth.2017.06.004
関連リンク : http://hdl.handle.net/10069/37800
権利: © 2017, The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
資料タイプ: Thesis or Dissertation
原稿種類: ETD
出現コレクション:110 学位論文

引用URI : http://hdl.handle.net/10069/37828



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