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Cyclic mechanical stretch contributes to network development of osteocyte-like cells with morphological change and autophagy promotion but without preferential cell alignment in rat


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タイトル: Cyclic mechanical stretch contributes to network development of osteocyte-like cells with morphological change and autophagy promotion but without preferential cell alignment in rat
その他のタイトル: 規則的な機械的伸展刺激による骨細胞様細胞ネットワークの発達には細胞形態の変化とオートファジーの亢進を 伴うが、細胞の優先配向は伴わない
著者: 稲葉, 菜緒
著者(別表記) : Inaba, Nao
発行日: 2017年 9月 6日
出版者: Elsevier B.V.
引用: Nagasaki University (長崎大学), 博士(歯学) (2017-09-06)
抄録: Osteocytes play important roles in controlling bone quality as well as preferential alignment of biological apatite c-axis/collagen fibers. However, the relationship between osteocytes and mechanical stress remains unclear due to the difficulty of three-dimensional (3D) culture of osteocytes in vitro. The aim of this study was to investigate the effect of cyclic mechanical stretch on 3D-cultured osteocyte-like cells. Osteocyte-like cells were established using rat calvarial osteoblasts cultured in a 3D culture system. Cyclic mechanical stretch (8% amplitude at a rate of 2 cycles min−1) was applied for 24, 48 and 96 consecutive hours. Morphology, cell number and preferential cell alignment were evaluated. Apoptosis- and autophagy-related gene expression levels were measured using quantitative PCR. 3D-cultured osteoblasts became osteocyte-like cells that expressed osteocyte-specific genes such as Dmp1, Cx43, Sost, Fgf23 and RANKL, with morphological changes similar to osteocytes. Cell number was significantly decreased in a time-dependent manner under non-loaded conditions, whereas cyclic mechanical stretch significantly prevented decreased cell numbers with increased expression of anti-apoptosis-related genes. Moreover, cyclic mechanical stretch significantly decreased cell size and ellipticity with increased expression of autophagy-related genes, LC3b and atg7. Interestingly, preferential cell alignment did not occur, irrespective of mechanical stretch. These findings suggest that an anti-apoptotic effect contributes to network development of osteocyte-like cells under loaded condition. Spherical change of osteocyte-like cells induced by mechanical stretch may be associated with autophagy upregulation. Preferential alignment of osteocytes induced by mechanical load in vivo may be partially predetermined before osteoblasts differentiate into osteocytes and embed into bone matrix.
記述: 長崎大学学位論文 学位記番号:博(医歯薬)甲第991号 学位授与年月日:平成29年9月6日 / Author: Nao Inaba, Shinichiro Kuroshima, Yusuke Uto, Muneteru Sasaki, Takashi Sawase / Citation: Biochemistry and Biophysics Reports, 11, pp.191-197; 2017
キーワード: Mechanical stimulation / Autophagy / Osteocyte-like cells / Cell morphology / Preferential cell alignment / Apoptosis
URI: http://hdl.handle.net/10069/37890
ISSN: 24055808
DOI: 10.1016/j.bbrep.2017.04.018
関連リンク : http://hdl.handle.net/10069/37802
権利: © 2017 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/).
資料タイプ: Thesis or Dissertation
原稿種類: ETD
出現コレクション:110 学位論文

引用URI : http://hdl.handle.net/10069/37890

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