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Determination of Tanshinones in Danshen (Salvia miltiorrhiza) by High-Performance Liquid Chromatography with Fluorescence Detection after pre-Column Derivatisation

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Title: Determination of Tanshinones in Danshen (Salvia miltiorrhiza) by High-Performance Liquid Chromatography with Fluorescence Detection after pre-Column Derivatisation
Authors: Kishikawa, Naoya / Yamanouchi, Akiko / El-Maghrabey, Mahmoud Hamed / Ohyama, Kaname / Kuroda, Naotaka
Issue Date: Feb-2018
Publisher: John Wiley and Sons Ltd.
Citation: Phytochemical Analysis, 29(1), pp.112-117; 2018
Abstract: Introduction: Tanshinones are a major class of bioactive ingredients in the traditional herbal medicines, Danshen (Salvia miltiorrhiza). A sensitive and reliable determination method for tanshinones is useful to ensure the quality of Danshen. Objective: To develop a sensitive and selective analytical method for tanshinones by high-performance liquid chromatography (HPLC) with fluorescence detection after pre-column derivatisation. Methodology: The proposed method depends on derivatisation reaction of tanshinones with 4-carbomethoxybenzaldehyde and ammonium acetate forming intensely fluorescent imidazole derivative. Results: The proposed method provided excellent sensitivity with the detection limits of 3.3 nM (66 fmol/injection), 3.2 nM (64 fmol/injection) and 2.0 nM (40 fmol/injection) for cryptotanshinone, tanshinone I and tanshinone IIA, respectively, without the necessity of complicated instrumentations. The developed method is successfully applied to quantify the contents of tanshinones in Danshen. Conclusion: The developed method is the first analytical method for tanshinones by fluorescence detection. Since the derivatisation reaction is selective for the o-quinone structure of tanshinone, the developed method will become a suitable mean for the discovering of tanshinone type diterpenoids from herbal samples.
Keywords: fluorescence detection / fluorogenic derivatisation / high-performance liquid chromatography / Salvia miltiorrhiza / tanshinone
URI: http://hdl.handle.net/10069/38343
ISSN: 09580344
DOI: 10.1002/pca.2719
Rights: © 2017 John Wiley & Sons, Ltd. This is the peer reviewed version of the following article: Phytochemical Analysis, 29(1), pp.112-117; 2018, which has been published in final form at http://dx.doi.org/10.1002/pca.2719. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/38343

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