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Determining Transgene Expression Characteristics Using a Suction Device with Multiple Hole Adjusting a Left Lateral Lobe of the Mouse Liver


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タイトル: Determining Transgene Expression Characteristics Using a Suction Device with Multiple Hole Adjusting a Left Lateral Lobe of the Mouse Liver
著者: Haraguchi, Ayana / Fuchigami, Yuki / Kawaguchi, Maho / Fumoto, Shintaro / Ohyama, Kaname / Shimizu, Kazunori / Hagimori, Masayori / Kawakami, Shigeru
発行日: 2018年 6月 1日
出版者: Pharmaceutical Society of Japan / 日本薬学会
引用: Biological and Pharmaceutical Bulletin, 41(6), pp.944-950; 2018
抄録: We developed a tissue suction-mediated transfection method (suction method) as a relatively reliable and less invasive technique for in vivo transfection. In this study, we determined hepatic transgene expression characteristics in the mouse liver, using a suction device, collecting information relevant to gene therapy and gene functional analysis by the liver suction method. To achieve high transgene expression levels, we developed a suction device with four holes (multiple hole device) and applied it to the larger portion of the left lateral lobe of the mouse liver. Hepatic transfection with physical stimuli was potentially controlled by activator protein-1 (AP-1) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). We examined the spatial distribution of transgene expression in the suctioned lobe by 2-dimensional imaging with histo-chemical staining and 3-dimensional multicolor deep imaging with tissue clearing methods. Through monitoring spatial distribution of transgene expression, the liver suction method was used to efficiently transfect extravascular hepatocytes in the suction-deformable upper lobe of the liver. Moreover, long-term transgene expression, at least 14d, was achieved with the liver suction method when cytosine-phosphate-guanine (CpG)-free plasmid DNA was applied.
キーワード: gene delivery / hepatocyte / naked plasmid DNA / liver
URI: http://hdl.handle.net/10069/38374
ISSN: 09186158
DOI: 10.1248/bpb.b18-00094
権利: © 2018 The Pharmaceutical Society of Japan.
資料タイプ: Journal Article
原稿種類: publisher
出現コレクション:110 学術雑誌論文

引用URI : http://hdl.handle.net/10069/38374

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