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Intrahepatic MicroRNA Profile of Liver Transplant Recipients with Hepatitis C Virus Co-Infected with Human Immunodeficiency Virus


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Title: Intrahepatic MicroRNA Profile of Liver Transplant Recipients with Hepatitis C Virus Co-Infected with Human Immunodeficiency Virus
Authors: Miyaaki, Hisamitsu / Takatsuki, Mitsuhisa / Ichikawa, Tatsuki / Hidaka, Masaaki / Soyama, Akihiko / Ohdan, Hideki / Inomata, Yukihiro / Uemoto, Shinji / Kokudo, Norihiro / Nakao, Kazuhiko / Eguchi, Susumu
Issue Date: 24-Nov-2017
Publisher: International Scientific Information, Inc.
Citation: Annals of Transplantation, 22, pp.701-706; 2017
Abstract: Background: In patients with human immunodeficiency virus (HIV) and hepatitis C virus (HCV) co-infection, HIV can modulate HCV replication and immune response as well as accelerate liver fibrosis. The role of miRNA in HIV/HCV co-infection is not fully elucidated. The aim of this study was to examine the differential expression of miRNAs in the liver. Material/Methods: Thirteen patients who had undergone a liver transplant (7 HCV-infected and 6 HIV/HCV-co-infected patients) were examined using a miRNA array containing 1347 human miRNAs. To confirm the microarray results, data for 20 patients (10 HCV-infected and 10 HIV/HCV-co-infected) were validated using real-time polymerase chain reaction probing for miR101b, miR149, and miR200c. This miRNA was selected based on microarray results and its biological significance in liver fibrosis. Results: Microarray analysis revealed 22 miRNAs that were differentially expressed in the HIV/HCV-co-infected group compared to the HCV-infected group (p<0.05). The expression of miR-101b and miR149 was significantly decreased in the HIV/HCV-co-infected group compared to that in the HCV-infected group (miR101b, 0.103±0.09 vs. 0.0157±0.0093, p=0.007; miR149, 0.152±0.159 vs. 0.0192±0.015, p=0.025). Conclusions: HIV/HCV co-infection may promote liver fibrosis by modulating miRNA expression.
Keywords: Hepatitis / Hepatitis C antibodies / Human / Liver cirrhosis / Viral
URI: http://hdl.handle.net/10069/38379
ISSN: 14259524
DOI: 10.12659/AOT.906236
Rights: © Annals of Transplantation, 2017. This paper has been published under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) allowing to download articles and share them with others as long as they credit the authors and the publisher, but without permission to change them in any way or use them commercially.
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/38379

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