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Inhibitory effect of sulphated polysaccharide porphyran (isolated from Porphyra yezoensis) on RANKL-induced differentiation of RAW264.7 cells into osteoclasts


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タイトル: Inhibitory effect of sulphated polysaccharide porphyran (isolated from Porphyra yezoensis) on RANKL-induced differentiation of RAW264.7 cells into osteoclasts
著者: Ueno, Mikinori / Cho, Kichul / Isaka, Shogo / Nishiguchi, Tomoki / Yamaguchi, Kenichi / Kim, Daekyung / Oda, Tatsuya
発行日: 2018年 3月
出版者: John Wiley and Sons Ltd
引用: Phytotherapy Research, 32(3), pp.452-458; 2018
抄録: Safe and efficient therapeutic agents for bone diseases are required in natural sources. We previously found that edible seaweed-derived polysaccharide porphyran exhibited anti-inflammatory effects through the down regulation of nuclear factor-κB. The aim of this study was to investigate the availability of porphyran as a therapeutic agent for bone diseases. The effects of porphyran on receptor activator of nuclear factor κB ligand (RANKL)-induced osteoclastogenesis in RAW264.7 cells were examined. Porphyran suppressed RANKL-induced osteoclast formation in a concentration-dependent manner (6.25–50 μg/ml) without any cytotoxic effects. Furthermore, real-time polymerase chain reaction analyses indicated that porphyran at 50 μg/ml significantly attenuated the RANKL-induced increase in the mRNA levels of osteoclastogenesis-related marker genes such as nuclear factor of activated T cells, tartrate-resistant acid phosphatase, cathepsin K, and matrix metalloproteinase-9 in RAW264.7 cells. To our knowledge, this is the first report showing that edible-seaweed-derived polysaccharide porphyran can suppress RANKL-induced osteoclastogenesis. Our results suggest that porphyran can be used as a safe therapeutic agent to improve osteoclast-related pathological conditions.
キーワード: osteoclast / Porphyra yezoensis / porphyran / RANKL / RAW264.7 cells
URI: http://hdl.handle.net/10069/38415
ISSN: 0951418X
DOI: 10.1002/ptr.5988
権利: © 2017 John Wiley & Sons, Ltd. This is the peer reviewed version of the following article: Phytotherapy Research, 32(3), pp.452-458; 2018, which has been published in final form at http://dx.doi.org/10.1002/ptr.5988. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
資料タイプ: Journal Article
原稿種類: author
出現コレクション:080 学術雑誌論文

引用URI : http://hdl.handle.net/10069/38415

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