DSpace university logo mark
Japanese | English 

NAOSITE : Nagasaki University's Academic Output SITE > 050 薬学部 > 050 学術雑誌論文 >

Ecto-F0/F1 ATPase as a novel candidate of prothymosin α receptor

ファイル 記述 サイズフォーマット
EOBT18_89.pdf538.95 kBAdobe PDF公開予定日: 2019-07-31

タイトル: Ecto-F0/F1 ATPase as a novel candidate of prothymosin α receptor
著者: Ueda, Hiroshi / Matsunaga, Hayato / Matsushita, Yosuke / Maeda, Shiori / Iwamoto, Ryusei / Yokoyama, Shigeyuki / Shirouzu, Mikako
発行日: 2018年 7月31日
出版者: Taylor and Francis Ltd
引用: Expert Opinion on Biological Therapy, 18(suppl 1), pp.89-94; 2018
抄録: Objectives: Prothymosin α (ProTα) was reported to inhibit the neuronal necrosis by facilitating the plasma membrane localization of endocytosed glucose transporter 1/4 through an activation of putative Gi-coupled receptor. The present study aims to identify a novel ProTα target, which may lead to an activation of Gi-coupled receptor. Methods: We used Gi-rich lipid rafts fraction of retinal cell line N18-RE-105 cells for affinity cross-linking. The biological confirmation that F0/F1 ATPase is a target protein complex was performed by cell-free experiments using ELISA-based binding assay, surface plasmon resonance assay and quartz crystal microbalance assay, and cell-based experiments to measure extracellular ATP level in the HUVECs culture. Results: From the cross-linking study and above-mentioned protein-protein interaction assays, ATP5A1 and ATP5B, F1 ATPase subunits were found to ProTα binding target proteins. In the culture of HUVEC cells, furthermore, ProTα increased the extracellular ATP levels in a reversible manner by anti-ATP5A1- and ATP5B-antibodies. Conclusion: The present study suggests that ProTα may activate ecto-F0/F1 ATPase and produced ATP. This study leads to next subjects whether produced ATP and its metabolites, ADP or adenosine may activate corresponding Gi-coupled receptors.
キーワード: ecto-F0/F1 ATPase / HUVEC / N18-RE-105 cells / protein-protein interaction / Prothymosin α
URI: http://hdl.handle.net/10069/38564
ISSN: 14712598
DOI: 10.1080/14712598.2018.1454427
権利: © 2018 Informa UK Limited, trading as Taylor & Francis Group. This is an Accepted Manuscript of an article published by Taylor & Francis in Expert Opinion on Biological Therapy on 2018-07-31, available online: http://www.tandfonline.com/.10.1080/14712598.2018.1454427
資料タイプ: Journal Article
原稿種類: author
出現コレクション:050 学術雑誌論文

引用URI : http://hdl.handle.net/10069/38564



Valid XHTML 1.0! Copyright © 2006-2015 長崎大学附属図書館 - お問い合わせ Powerd by DSpace