DSpace university logo mark
詳細検索
Japanese | English 

NAOSITE : Nagasaki University's Academic Output SITE > 050 薬学部 > 050 学術雑誌論文 >

Ecto-F0/F1 ATPase as a novel candidate of prothymosin α receptor


ファイル 記述 サイズフォーマット
EOBT18_89.pdf538.95 kBAdobe PDF公開予定日: 2019-07-31

タイトル: Ecto-F0/F1 ATPase as a novel candidate of prothymosin α receptor
著者: Ueda, Hiroshi / Matsunaga, Hayato / Matsushita, Yosuke / Maeda, Shiori / Iwamoto, Ryusei / Yokoyama, Shigeyuki / Shirouzu, Mikako
発行日: 2018年 7月31日
出版者: Taylor and Francis Ltd
引用: Expert Opinion on Biological Therapy, 18(suppl 1), pp.89-94; 2018
抄録: Objectives: Prothymosin α (ProTα) was reported to inhibit the neuronal necrosis by facilitating the plasma membrane localization of endocytosed glucose transporter 1/4 through an activation of putative Gi-coupled receptor. The present study aims to identify a novel ProTα target, which may lead to an activation of Gi-coupled receptor. Methods: We used Gi-rich lipid rafts fraction of retinal cell line N18-RE-105 cells for affinity cross-linking. The biological confirmation that F0/F1 ATPase is a target protein complex was performed by cell-free experiments using ELISA-based binding assay, surface plasmon resonance assay and quartz crystal microbalance assay, and cell-based experiments to measure extracellular ATP level in the HUVECs culture. Results: From the cross-linking study and above-mentioned protein-protein interaction assays, ATP5A1 and ATP5B, F1 ATPase subunits were found to ProTα binding target proteins. In the culture of HUVEC cells, furthermore, ProTα increased the extracellular ATP levels in a reversible manner by anti-ATP5A1- and ATP5B-antibodies. Conclusion: The present study suggests that ProTα may activate ecto-F0/F1 ATPase and produced ATP. This study leads to next subjects whether produced ATP and its metabolites, ADP or adenosine may activate corresponding Gi-coupled receptors.
キーワード: ecto-F0/F1 ATPase / HUVEC / N18-RE-105 cells / protein-protein interaction / Prothymosin α
URI: http://hdl.handle.net/10069/38564
ISSN: 14712598
DOI: 10.1080/14712598.2018.1454427
権利: © 2018 Informa UK Limited, trading as Taylor & Francis Group. This is an Accepted Manuscript of an article published by Taylor & Francis in Expert Opinion on Biological Therapy on 2018-07-31, available online: http://www.tandfonline.com/.10.1080/14712598.2018.1454427
資料タイプ: Journal Article
原稿種類: author
出現コレクション:050 学術雑誌論文

引用URI : http://hdl.handle.net/10069/38564

このリポジトリに保管されている文献はすべて著作権により保護されています。
印刷やダウンロード等データの複製は、調査研究・教育または学習を目的とする場合に限定されます。

 

Valid XHTML 1.0! Copyright © 2006-2015 長崎大学附属図書館 - お問い合わせ Powerd by DSpace