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Identification of a major glucose transporter in Flavobacterium johnsoniae: Inhibition of F. johnsoniae colony spreading by glucose uptake

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Title: Identification of a major glucose transporter in Flavobacterium johnsoniae: Inhibition of F. johnsoniae colony spreading by glucose uptake
Authors: Imamura, Keigo / Sato, Keiko / Narita, Yuka / Kondo, Yoshio / Nakane, Daisuke / Naito, Mariko / Fujiwara, Taku / Nakayama, Koji
Issue Date: Aug-2018
Publisher: John Wiley & Sons Australia, Ltd
Citation: Microbiology and Immunology, 62(8), pp.507-516; 2018
Abstract: Many members of the phylum Bacteroidetes, such as Flavobacterium johnsoniae, can glide over a solid surface: an ability called gliding motility. It can be usually observed on agar plates as thin, flat, spreading colonies with irregular, feathery edges; this phenomenon is called colony spreading. Colony spreading of F. johnsoniae on 1.5% agar plates containing poor nutrients is dose-dependently inhibited by addition of D-glucose, as previously reported. Accordingly, here, we created mutants (by transposon mutagenesis) that partially suppressed glucose-mediated inhibition of colony spreading. Among the isolates, we found that one had a transposon insertion in Fjoh_4565, tentatively named mfsA, which encodes a major facilitator superfamily (MFS) transporter previously shown to be required for growth on glucose, N-acetyl-glucosamine, and chitin. We constructed an mfsA deletion mutant and found that the mutant showed no glucose-mediated acceleration of growth or glucose uptake. The mfsA gene complemented the phenotype of a glucose-negative Escherichia coli. These results suggest that the mfsA gene encodes the sole MFS transporter of glucose in F. johnsoniae and that glucose uptake is partially required for the glucose-mediated inhibition of F. johnsoniae colony spreading.
Keywords: colony spreading / gliding motility / glucose uptake / major facilitator superfamily transporter
URI: http://hdl.handle.net/10069/38574
ISSN: 03855600
DOI: 10.1111/1348-0421.12633
Rights: © 2018 The Societies and John Wiley & Sons Australia, Ltd. This is the peer reviewed version of the following article:Microbiology and Immunology, 62(8), pp.507-516; 2018, which has been published in final form at http://dx.doi.org/10.1111/1348-0421.12633 .This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/38574

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