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The effect of transient oxygenation on stem cell mobilization and ischemia/reperfusion heart injury


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タイトル: The effect of transient oxygenation on stem cell mobilization and ischemia/reperfusion heart injury
その他のタイトル: 高濃度酸素吸入が幹細胞動員と心臓虚血再灌流傷害に与える影響
著者: 矢野, 倫太郎
著者(別表記) : Yano, Rintaro
発行日: 2018年 9月 5日
出版者: Public Library of Science
引用: Nagasaki University (長崎大学), 博士(医学) (2018-09-05)
抄録: For general anesthesia, pre-oxygenation is routinely performed prior to intubation. It is well-known that ischemic/hypoxic preconditioning induces stem cell mobilization and protects against ischemia/reperfusion (I/R) injury. In this study, we investigated the effect of transient oxygenation on stem cell mobilization and I/R injury of the heart. Mice were exposed to 100% oxygen for 5 or 20 minutes. We evaluated the number of c-kit+ stem/progenitor cells and the levels of SDF-1α and VEGF in peripheral blood at 1, 3, 6, and 24 hours after oxygenation. We also induced I/R injury of the heart at 3 hours post-oxygenation for 5 minutes and then examined stem cell recruitment and fibrotic changes in the heart 3 or 14 days later. The number of c-kit+ cells in peripheral blood was significantly increased at 1 or 24 hours after oxygenation for either 5 or 20 minutes. Oxygenation for 5 or 20 minutes did not significantly change the SDF-1α level measured in plasma. However, the plasma VEGF level was decreased at 3 hours post-oxygenation for 20 minutes (p = 0.051). Oxygenation for 5 minutes did not significantly alter the fibrotic area or cell apoptosis. Although oxygenation for 5 minutes increased the number of c-kit+ cells in hearts damaged by I/R injury, this difference was not significant between groups due to large variation between individuals (p = 0.14). Although transient oxygenation induces stem cell mobilization, it does not appear to protect against I/R injury of the heart in mice.
記述: 長崎大学学位論文 学位記番号:博(医歯薬)甲第1088号 学位授与年月日:平成30年9月5日 / Author: Rintaro Yano, Chiaki Inadomi, Lan Luo, Shinji Goto, Tetsuya Hara, Tao-Sheng Li / Citation: PLOS ONE, 13(2), e0192733; 2018
URI: http://hdl.handle.net/10069/38575
DOI: 10.1371/journal.pone.0192733
権利: © 2018 Yano et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
資料タイプ: Thesis or Dissertation
原稿種類: ETD
出現コレクション:110 学位論文

引用URI : http://hdl.handle.net/10069/38575

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