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Critical role of Erythrocyte Binding-Like protein of the rodent malaria parasite Plasmodium yoelii to establish an irreversible connection with the erythrocyte during invasion


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タイトル: Critical role of Erythrocyte Binding-Like protein of the rodent malaria parasite Plasmodium yoelii to establish an irreversible connection with the erythrocyte during invasion
その他のタイトル: ネズミマラリア原虫Plasmodium yoeliiのErythrocyte Binding-Likeタンパク質は赤血球侵入過程における不可逆的結合形成に重要である
著者: 外川, 裕人
著者(別表記) : Kegawa, Yuto
発行日: 2018年 9月 5日
出版者: Elsevier B.V.
引用: Nagasaki University (長崎大学), 博士(医学) (2018-09-05)
抄録: Plasmodium malaria parasites multiply within erythrocytes and possess a repertoire of proteins whose function is to recognize and invade these vertebrate host cells. One such protein involved in erythrocyte invasion is the micronemal protein, Erythrocyte Binding-Like (EBL), which has been studied as a potential target of vaccine development in Plasmodium vivax (PvDBP) and Plasmodium falciparum (EBA-175). In the rodent malaria parasite model Plasmodium yoelii, specific substitutions in the EBL regions responsible for intracellular trafficking (17XL parasite line) or receptor recognition (17X1.1pp. parasite line), paradoxically increase invasion ability and virulence rather than abolish EBL function. Attempts to disrupt the ebl gene locus in the 17XL and 17XNL lines were unsuccessful, suggesting EBL essentiality. To understand the mechanisms behind these potentially conflicting outcomes, we generated 17XL-based transfectants in which ebl expression is suppressed with anhydrotetracycline (ATc) and investigated merozoite behavior during erythrocyte invasion. In the absence of ATc, EBL was secreted to the merozoite surface, whereas following ATc administration parasitemia was negligible in vivo. Merozoites lacking EBL were unable to invade erythrocytes in vitro, indicating that EBL has a critical role for erythrocyte invasion. Quantitative time-lapse imaging revealed that with ATc administration a significant number of merozoites were detached from the erythrocyte after the erythrocyte deformation event and no echinocytosis was observed, indicating that EBL is required for merozoites to establish an irreversible connection with erythrocytes during invasion.
記述: 長崎大学学位論文 学位記番号:博(医歯薬)甲第1093号 学位授与年月日:平成30年9月5日 / Author: Yuto Kegawa, Masahito Asada, Takahiro Ishizaki, Kazuhide Yahata, Osamu Kaneko / Citation: Parasitology International, 67(6), pp.706-714; 2018
キーワード: EBL / Erythrocyte / Invasion / Malaria / Plasmodium yoelii / Time-lapse imaging
URI: http://hdl.handle.net/10069/38580
ISSN: 13835769
DOI: 10.1016/j.parint.2018.07.006
権利: © 2018 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/).
資料タイプ: Thesis or Dissertation
原稿種類: ETD
出現コレクション:110 学位論文

引用URI : http://hdl.handle.net/10069/38580

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