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Methotrexate-Coated Complexes of Plasmid DNA and Polyethylenimine for Gene Delivery

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タイトル: Methotrexate-Coated Complexes of Plasmid DNA and Polyethylenimine for Gene Delivery
著者: Kodama, Yukinobu / Noda, Ryo / Sato, Kayoko / Harasawa, Hitomi / Kurosaki, Tomoaki / Nakagawa, Hiroo / Nakamura, Tadahiro / Kitahara, Takashi / Muro, Takahiro / Sasaki, Hitoshi
発行日: 2018年10月 1日
出版者: 日本薬学会 / Pharmaceutical Society of Japan 
引用: Biological and Pharmaceutical Bulletin, 41(10), pp.1537-1542; 2018  
抄録: Folate receptors are overexpressed on the surface cancer cells. We successfully constructed a new gene delivery vector of methotrexate (MTX)-coated plasmid DNA-polyethylenimine (pDNA-PEI) complexes (PEI complexes) by electrostatic binding. The stable anionic nanoparticle was optimized at MTX charge ratios of 120 or more. pDNA-PEI-MTX complexes (MTX complexes) demonstrated gene expression efficiency as high as cationic pDNA-PEI complexes in the mouse melanoma cell line, B16-F10. The MTX complexes were taken up by the cell-specific uptake mechanisms via the folate receptor. MTX-coated complexes are useful as endocytosis ligands. The MTX120 complexes exhibited no blood aggregation. The transgene efficiency of MTX120 complexes in the liver and spleen after their intravenous administration was higher than that of PEI complexes. Therefore, MTX complexes are expected as a new gene vector in the future.
キーワード: DNA / gene delivery / methotrex / ateternary complex
URI: http://hdl.handle.net/10069/38631
ISSN: 09186158
DOI: 10.1248/bpb.b18-00144
権利: © 2018 The Pharmaceutical Society of Japan
資料タイプ: Journal Article
原稿種類: publisher
出現コレクション:130 学術雑誌論文

引用URI : http://hdl.handle.net/10069/38631



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