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Genetic characterization of Lassa virus strains isolated from 2012 to 2016 in southeastern Nigeria.


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タイトル: Genetic characterization of Lassa virus strains isolated from 2012 to 2016 in southeastern Nigeria.
その他のタイトル: 2012年から2016年にかけてナイジェリア南東部で検出されたラッサウイルスの遺伝学的解析
著者: OLONINIYI, OLAMIDE KOLAWOLE
著者(別表記) : オロニニイ, オラミデ コラウォレ
発行日: 2018年12月 5日
出版者: Public Library of Science
引用: Nagasaki University (長崎大学), 博士(医学) (2018-12-05)
抄録: Lassa virus (LASV) is endemic in parts of West Africa where it causes Lassa fever (LF), a viral hemorrhagic fever with frequent fatal outcomes. The diverse LASV strains are grouped into six major lineages based on the geographical location of the isolated strains. In this study, we have focused on the lineage II strains from southern Nigeria. We determined the viral sequences from positive cases of LF reported at tertiary hospitals in Ebonyi and Enugu between 2012 and 2016. Reverse transcription-polymerase chain reaction (RT-PCR) showed that 29 out of 123 suspected cases were positive for the virus among which 11 viral gene sequences were determined. Phylogenetic analysis of the complete coding sequences of the four viral proteins revealed that lineage II strains are broadly divided into two genetic clades that diverged from a common ancestor 195 years ago. One clade, consisting of strains from Ebonyi and Enugu, was more conserved than the other from Irrua, although the four viral proteins were evolving at similar rates in both clades. These results suggested that the viruses of these clades have been distinctively evolving in geographically separate parts of southern Nigeria. Furthermore, the epidemiological data of the 2014 outbreak highlighted the role of human-to-human transmission in this outbreak, which was supported by phylogenetic analysis showing that 13 of the 16 sequences clustered together. These results provide new insights into the evolution of LASV in southern Nigeria and have important implications for vaccine development, diagnostic assay design, and LF outbreak management.
記述: 長崎大学学位論文 学位記番号:博(医歯薬)甲第1107号 学位授与年月日:平成30年12月5日 / Author: Olamide K. Oloniniyi, Uche S. Unigwe, Sayaka Okada, Mayuko Kimura, Shota Koyano, Yukiko Miyazaki, Michael O. Iroezindu, Nnenna A. Ajayi, Chinedu M. Chukwubike, Nneka M. Chika-Igwenyi, Anne C. Ndu, Damian U. Nwidi, Haruka Abe, Shuzo Urata, Yohei Kurosaki, Jiro Yasuda / Citation: PLoS Neglected Tropical Diseases, 12(11), e0006971; 2018
URI: http://hdl.handle.net/10069/38729
DOI: 10.1371/journal.pntd.0006971
関連リンク : http://hdl.handle.net/10069/38743
権利: © 2018 Oloniniyi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
資料タイプ: Thesis or Dissertation
原稿種類: ETD
出現コレクション:110 学位論文

引用URI : http://hdl.handle.net/10069/38729

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