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Six species of nontuberculous mycobacteria carry non-identical 16S rRNA gene copies


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タイトル: Six species of nontuberculous mycobacteria carry non-identical 16S rRNA gene copies
著者: Takeda, Keita / Chikamatsu, Kinuyo / Igarashi, Yuriko / Morishige, Yuta / Murase, Yoshiro / Aono, Akio / Yamada, Hiroyuki / Takaki, Akiko / Mitarai, Satoshi
発行日: 2018年12月
出版者: Elsevier B.V.
引用: Journal of Microbiological Methods, 155, pp.34-36; 2018
抄録: Nontuberculous mycobacteria (NTM) can carry two or more 16S rRNA gene copies that are, in some instances, non-identical. In this study, we used a combined cloning and sequencing approach to analyze 16S rRNA gene sequences of six NTM species, Mycobacterium cosmeticum, M. pallens, M. hodleri, M. crocinum, M. flavescens, and M. xenopi. Our approach facilitated the identification of two distinct gene copies in each species. The two M. cosmeticum genes had a single nucleotide difference, whereas two nucleotide polymorphisms were identified in M. hodleri, M. flavescens, and M. xenopi. M. pallens had a difference in four nucleotides and M. crocinum — in 23 nucleotides. Thus, we showed that the six NTM species possess at least two non-identical 16S rRNA gene copies. The full-length sequences of the intraspecies 16S rRNA variants will facilitate NTM identification and sequence analysis of specimens or other samples.
キーワード: 16S rRNA / Gene copy / Mycobacterium / Nontuberculous mycobacteria
URI: http://hdl.handle.net/10069/38730
ISSN: 01677012
DOI: 10.1016/j.mimet.2018.08.012
権利: © 2018, Elsevier. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
資料タイプ: Journal Article
原稿種類: author
出現コレクション:110 学術雑誌論文

引用URI : http://hdl.handle.net/10069/38730

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