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Comparison of enzyme-linked immunosorbent assay systems using rift valley fever virus nucleocapsid protein and inactivated virus as antigens

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タイトル: Comparison of enzyme-linked immunosorbent assay systems using rift valley fever virus nucleocapsid protein and inactivated virus as antigens
著者: Yu, Fuxun / Adungo, Ferdinard / Konongoi, Samson Limbaso / Inoue, Shingo / Sang, Rosemary / Ashur, Salame / Kwallah, Allan ole / Uchida, Leo / Buerano, Corazon C / Mwau, Matilu / Zha, Yan / Nie, Yingjie / Morita, Kouichi
発行日: 2018年11月22日
出版者: Bio Med Central
引用: Virology Journal, 15(1), 178; 2018
抄録: BACKGROUND: Rift Valley Fever(RVF)is a mosquito-borne viral zoonosis.To detect RVF virus(RVFV)infection,indirect immunoglobulin G(IgG)and immunoglobulin M(IgM)enzyme linked immunosorbent assays(ELISAs)which utilize recombinant RVFV nucleocapsid(RVFV-N)protein as assay antigen, have reportedly been used, however, there is still a need to develop more sensitive and specific methods of detection. METHODS: RVFV-N protein was expressed in Escherichia coli (E. coli) and purified by histidine-tag based affinity chromatography. This recombinant RVFV-N (rRVFV-N) protein was then used as antigen to develop an IgG sandwich ELISA and IgM capture ELISAs for human sera. Ninety six serum samples collected from healthy volunteers during the RVF surveillance programme in Kenya in 2013, and 93 serum samples collected from RVF-suspected patients during the 2006-2007 RVF outbreak in Kenya were used respectively, to evaluate the newly established rRVFV-N protein-based IgG sandwich ELISA and IgM capture ELISA systems in comparison with the inactivated virus-based ELISA systems. RESULTS: rRVFV-N protein-based-IgG sandwich ELISA and IgM capture ELISA for human sera were established.Both the new ELISA systems were in 100% concordance with the inactivated virus-based ELISA systems, with a sensitivity and specificity of 100%. CONCLUSIONS: Recombinant RVFV-N is a safe and affordable antigen for RVF diagnosis.Our rRVFV-N-based ELISA systems are safe and reliable tools for diagnosis of RVFV infection in humans and especially useful in large-scale epidemiological investigation and for application in developing countries.
キーワード: Expression / IgG sandwich ELISA / IgM capture ELISA / Nucleocapsid protein / RVFV
URI: http://hdl.handle.net/10069/38733
DOI: 10.1186/s12985-018-1071-y
権利: ©The Author(s). 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
資料タイプ: Journal Article
原稿種類: publisher
出現コレクション:120 学術雑誌論文

引用URI : http://hdl.handle.net/10069/38733



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