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Application of Non-Fluorescent Dyes to Assess the Antischistosomal Effect of Antimalarial Drugs on Schistosoma mansoni Adult Worms


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タイトル: Application of Non-Fluorescent Dyes to Assess the Antischistosomal Effect of Antimalarial Drugs on Schistosoma mansoni Adult Worms
著者: Mitsui, Yoshinori / Kato, Kentaro
発行日: 2018年 9月30日
出版者: 国立感染症研究所
引用: Japanese Journal of Infectious Diseases, 71(5), pp.382-387; 2018
抄録: The possible emergence of praziquantel (PZQ)-tolerant and/or-resistant schistosomes requires the study and development of new antischistosomal drugs as alternatives to PZQ. The present study investigates the capability of 3 dyes—methylene blue (MB), neutral red (NR), and trypan blue (TB)—to assess the in vitro antischistosomal effect of antimalarial drugs on Schistosoma mansoni adult worms. S. mansoni adult worms were incubated in the medium alone as the control or in the medium supplemented with 10 μg/ml primaquine (PQ), artesunate (AR), or amodiaquine (AQ) for 5 days. Viabili-ties of the worms were observed following staining with MB, NR, or TB. The disparity of MB and NR staining among male and female adult worms treated with PQ, AR, and AQ correlated with the various levels of damage to the male and female worms.Furthermore, the severity of the damage to the adult worms treated with the 3 drugs appeared to be reflected in the TB staining status. The results indicate that the 3 non-fluorescent dyes can serve as useful complementary tools to assess the antischistosomal effect of antimalarial drugs.
キーワード: methylene blue / neutral red / trypan blue / antimalarial drug / antischistosomal effect
URI: http://hdl.handle.net/10069/38735
ISSN: 13446304
DOI: 10.7883/yoken.JJID.2018.034
権利: © 2018 National Institute of Infectious Diseases
資料タイプ: Journal Article
原稿種類: publisher
出現コレクション:120 学術雑誌論文

引用URI : http://hdl.handle.net/10069/38735

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