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Biocompatible complex coated with glycosaminoglycan for gene delivery

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タイトル: Biocompatible complex coated with glycosaminoglycan for gene delivery
その他のタイトル: グリコサミノグリカンを用いた完全生体分解型遺伝子デリバリー製剤の開発
著者: 岩永, 真理恵
著者(別表記) : Iwanaga, Marie
発行日: 2017年 3月 8日
出版者: Taylor & Francis
引用: Nagasaki University (長崎大学), 博士(医学) (2017-03-08)
抄録: The purpose of this study was to develop a ternary complex of plasmid DNA (pDNA) electrostatically assembled with dendrigraft poly-l-lysine (DGL) and biodegradable glycosaminoglycan for effective and secure gene delivery. High gene expression of pDNA/DGL complex was confirmed with slight cytotoxicity and erythrocyte agglutination. Anionic ternary complexes of 55.4–223.8 nm were formed by the addition of a glycosaminoglycan such as chondroitin sulfate A (CS-A), chondroitin sulfate B (CS-B), chondroitin sulfate C (CS-C) or hyaluronic acid (HA). Using the cell line B16-F10, most of the ternary complexes showed only weak gene expression and little cytotoxicity, although the pDNA/DGL/CS-A complexes maintained a certain level of gene expression. In particular, the pDNA/DGL/CS-A8 complexes showed significantly higher gene expression than pDNA/DGL complexes in the presence of fetal bovine serum. Gene expression from the pDNA/DGL/CS-A8 complex was inhibited by a high concentration of CS-A and endocytosis inhibitors. After intravenous administration of the pDNA/DGL/CS-A8 complex and the pDNA/DGL complex into ddY mice, high gene expression was observed in the reticuloendothelial systems, the pDNA/DGL/CS-A complex is expected to be useful for gene therapy.
記述: 長崎大学学位論文 学位記番号:博(医歯薬)甲第944号 学位授与年月日:平成29年3月8日 / Author: Marie Iwanaga, Yukinobu Kodama, Takahiro Muro, Hiroo Nakagawa, Tomoaki Kurosaki, Kayoko Sato, Tadahiro Nakamura, Takashi Kitahara & Hitoshi Sasaki / Citation: Journal of Drug Targeting, 25(4), pp.370-378; 2017
キーワード: Dendrigraft poly-l-lysine / chondroitin sulfate / hyaluronic acid / biodegradable / glycosaminoglycan / gene therapy / drug delivery
URI: http://hdl.handle.net/10069/38753
ISSN: 1061186X
DOI: 10.1080/1061186X.2016.1274996
関連リンク : http://hdl.handle.net/10069/37402
権利: This is an Accepted Manuscript of an article published by Taylor & Francis in Journal of Drug Targeting on 12 Jan 2017, available online: http://www.tandfonline.com/10.1080/1061186X.2016.1274996
資料タイプ: Thesis or Dissertation
原稿種類: ETD
出現コレクション:110 学位論文

引用URI : http://hdl.handle.net/10069/38753



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