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クロマトグラフィー分離を基盤とした新しいプロテオーム解析による臨床薬学研究


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Title: クロマトグラフィー分離を基盤とした新しいプロテオーム解析による臨床薬学研究
Other Titles: Clinical Pharmaceutical Research Based on New Proteome Analysis Based on Chromatographic Separation
Authors: 大山, 要
Authors (alternative): Ohyama, Kaname
Issue Date: 1-Apr-2019
Publisher: 日本薬学会 / The Pharmaceutical Society of Japan
Citation: Yakugaku Zasshi, 139(4), pp.505-509; 2019
Abstract: Comprehensive identification of antigens in immune complexes (IC-antigens) is beneficial to provide insights into pathophysiology and could form the basis for novel diagnostic and treatment strategies for many immune-related diseases. Immune complexome analysis is a method for comprehensively identifying and profiling IC-antigens in biological fluids (such as serum and cerebrospinal fluid). We applied this strategy to the analysis of circulating ICs in autoimmune diseases (rheumatoid arthritis, Sjögren's syndrome, systemic scleroderma, and systemic lupus erythematosus), infectious diseases, and cancers. Fluorogenic derivatization-liquid chromatography-tandem mass spectrometry (FD-LC-MS/MS) consists of fluorogenic derivatization of proteins, followed by HPLC of the derivatized proteins, isolation of the proteins differentially expressed in a certain group, enzymatic digestion of the isolated proteins followed by LC-tandem MS using a database-searching algorithm for protein identification. We have applied this method to understand the cardioprotective effect of pre-administration of docetaxel in adriamycin/docetaxel combination anti-cancer therapy, and the cellular processes that are affected by non-steroidal anti-inflammatory drugs (NSAIDs) in mouse stomach tissue during ulcer formation.
Keywords: autoimmune disease / cancer / fluorogenic derivatization-liquid chromatography-tandem mass spectrometry / immune complex / immune complexome analysis / toxicoproteomics
URI: http://hdl.handle.net/10069/38995
ISSN: 00316903
DOI: 10.1248/yakushi.18-00167
Rights: © 2019 The Pharmaceutical Society of Japan
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/38995

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