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Ameliorated healing of biliary anastomosis by autologous adipose-derived stem cell sheets


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Title: Ameliorated healing of biliary anastomosis by autologous adipose-derived stem cell sheets
Authors: Hara, Takanobu / Soyama, Akihiko / Adachi, Toshiyuki / Kobayashi, Shinichiro / Sakai, Yusuke / Maruya, Yasuhiro / Kugiyama, Tota / Hidaka, Masaaki / Okada, Satomi / Hamada, Takashi / Maekawa, Kyoichiro / Ono, Shinichiro / Adachi, Tomohiko / Takatsuki, Mitsuhisa / Eguchi, Susumu
Issue Date: 17-Jan-2020
Publisher: 日本再生医療学会 / The Japanese Society for Regenerative Medicine
Citation: Regenerative Therapy, 14, pp.79-86; 2020
Abstract: Introduction: Cell sheets consisting of adipose-derived stem cells (ADSCs) have been reported to be effective for wound healing. We conducted this study to clarify the efficacy of ADSC sheets in wound healing at the duct-to-duct biliary anastomotic site in pigs. Methods: Eleven female pigs (20–25 kg) were divided into two groups: biliary anastomosis with an ADSC sheet (n = 6) or without an ADSC sheet (n = 5). To follow the transplanted ADSCs, PKH26GL-labeled sheets were used in one of the ADSC pigs. Two weeks prior to laparotomy, ADSCs were isolated from the lower abdominal subcutaneous adipose tissue. After three passages, ADSCs were seeded on temperature-responsive culture dishes and collected as cell sheets. ADSC sheets were gently transplanted on the anastomotic site. We evaluated specimens by PKH26GL labeling, macroscopic changes, infiltration of inflammatory cells, and collagen content. Results: Labeled ADSCs remained around the bile duct wall. In the no-ADSC group, more adhesion developed at the hepatic hilum as observed during relaparotomy. Histopathological examination showed that the diameter and cross-sectional area of the bile duct wall were decreased in the ADSC group. In the no-ADSC group, a large number of inflammatory cells and more collagen fibers were identified in the bile duct wall. Conclusions: The present study demonstrated that autologous ADSC sheet transplantation reduced hypertrophic changes in the bile duct wall at the anastomotic site. A long-term follow-up is required to evaluate the efficacy of this mechanism in prevention of biliary anastomotic strictures.
Keywords: Adipose-derived stem cell / Cell sheet / Biliary anastomosis / Anastomotic healing
URI: http://hdl.handle.net/10069/39634
ISSN: 23523204
DOI: 10.1016/j.reth.2019.11.001
Rights: © 2020, The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/39634

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