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β-Eudesmol induces the expression of apoptosis pathway proteins in cholangiocarcinoma cell lines

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Title: β-Eudesmol induces the expression of apoptosis pathway proteins in cholangiocarcinoma cell lines
Authors: Karbwang, Juntra / Na-Bangchang, Kesara / Narahara, Chisato / Saeheng, Teerachat / Chaijaroenkul, Wanna / Dumre, ShyamPrakash
Issue Date: 20-Jan-2020
Publisher: Wolters Kluwer Medknow Publications
Citation: Journal of Research in Medical Sciences, 25(1), art.no.7; 2020
Abstract: Background: Cholangiocarcinoma (CCA) is a neglected disease prevalent in developing countries with high burden and mortality rate, and there is no effective treatment. We aimed to investigate β-eudesmol molecular target of action in human CCA cell lines using the selected key molecules of apoptotic pathways. Materials and Methods: Two CCA cell lines (HuH28 and HuCCT1) were assessed at different time points after β-eudesmol treatment for mRNA and protein expression profiles of caspase-3, -8, -9, p53, p21, Bcl-2, and Bax by real-time polymerase chain reaction and western blot, respectively. Results: β-eudesmol induced expressions of p21 and p53 in mRNA/protein level in HuH28 and HuCCT1 cells. These CCA cells also expressed caspase-3, -8, -9 and bax (mRNA and/or protein level) among others after β-eudesmol treatment indicating its role in both intrinsic and extrinsic caspase-dependent apoptotic pathways. Conclusion: The study demonstrated that β-eudesmol induced the expression of apoptosis pathway proteins, suggesting its potential role in promoting the caspase-dependent apoptotic pathway, and induction of the cell cycle arrest in CCA cell lines. β-eudesmol can be considered as a potential compound for further investigation as an anti-CCA agent.
Keywords: Apoptosis / cholangiocarcinoma / mode of action / molecular targets / β‑eudesmol
URI: http://hdl.handle.net/10069/39659
ISSN: 17351995
DOI: 10.4103/jrms.JRMS_291_19
Rights: © 2020 Journal of Research in Medical Sciences. This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/39659

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