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Tetrodotoxin functions as a stress relieving substance in juvenile tiger puffer Takifugu rubripes


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Title: Tetrodotoxin functions as a stress relieving substance in juvenile tiger puffer Takifugu rubripes
Authors: Amano, Masafumi / Amiya, Noriko / Takaoka, Minami / Sato, Haruka / Takatani, Tomohiro / Arakawa, Osamu / Sakakura, Yoshitaka
Issue Date: 5-Dec-2019
Publisher: Elsevier Ltd.
Citation: Toxicon, 171, pp.54-61; 2019
Abstract: We tested whether tetrodotoxin (TTX) functions as a stress relieving substance in puffer fish. We orally administered TTX to the juveniles of hatchery-reared non-toxic tiger puffer Takifugu rubripes and measured the effects of TTX on brain corticotropin-releasing hormone (CRH) mRNA expression and plasma cortisol levels in comparison with effects in non-toxic juveniles. Firstly, the reciprocal connections of CRH and adrenocorticotropic hormone (ACTH) were confirmed by dual-label immunohistochemistry. CRH-immunoreactive (ir) cell bodies were detected in the hypothalamus and CRH-ir fibers were observed to project to ACTH-ir cells in the rostral pars distalis of the pituitary. Next, a TTX-containing diet (2.35 mouse units (517 ng)/g diet) or a non-toxic diet were fed to the fish for 28 days under a recirculating system. Standard length and body weight became significantly larger in the TTX-treated group. The degree of loss of the caudal fin, which is an indicator of the degree of agonistic interactions, where high values show a higher loss of caudal fin of a fish due to nipping by other individuals, was significantly lower in the TTX-treated group. Relative CRH mRNA expression levels in the brain and cortisol levels in the plasma were significantly lower in the TTX-treated group. These results indicate that TTX functions as a stress relieving substance by affecting the CRH-ACTH-cortisol axis and reducing agonistic interactions in tiger puffer juveniles.
Keywords: TTX / CRH / ACTH / Cortisol / Stress / Tiger puffer
URI: http://hdl.handle.net/10069/39769
ISSN: 00410101
DOI: 10.1016/j.toxicon.2019.09.024
Rights: © 2019 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/39769

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