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Identification of a modori-inducing proteinase in the threadfin bream: Molecular cloning, tissue distribution and proteinase leakage from viscera during ice storage


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Title: Identification of a modori-inducing proteinase in the threadfin bream: Molecular cloning, tissue distribution and proteinase leakage from viscera during ice storage
Authors: Liu, Jin-Yang / Yoshida, Asami / Gao, Yi-Li / Shirota, Kazuya / Shiina, Yasuhiko / Osatomi, Kiyoshi
Issue Date: 4-Jun-2020
Publisher: Elsevier Ltd.
Citation: Food Chemistry, 330, art.no.127246; 2020
Abstract: Previously we purified and characterized a sarcoplasmic serine proteinase (SSP) from the belly muscle of the threadfin bream as a modori-inducing proteinase. In our attempt to clarify the structure and physiological functions of SSP, we successfully cloned the full-length cDNA of SSP (ORF 726 bp). The deduced amino acid sequence of SSP (241 residues) was highly homologous to fish trypsinogen. The distribution of SSP mRNA and the proteinase activity in the tissue indicated that SSP was mainly synthesized and existed in the digestive system under physiological conditions. After ice storage of the threadfin bream without gutting, a high SSP activity was detected only in the belly muscle because of SSP leaked from the viscera. Therefore, it is desirable to use edible proteinase inhibitor to inactivate the leaked SSP during production of surimi-based products or to take effective measures to prevent the proteinase leakage during post-harvest storage.
Keywords: Modori phenomenon / Molecular cloning / Proteinase leakage / Serine proteinase / Tissue distribution
URI: http://hdl.handle.net/10069/40066
ISSN: 03088146
DOI: 10.1016/j.foodchem.2020.127246
Rights: © 2020 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/40066

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