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Spontaneous Development of Dental Dysplasia in Aged Parp-1 Knockout Mice

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Title: Spontaneous Development of Dental Dysplasia in Aged Parp-1 Knockout Mice
Authors: Fujihara, Hisako / Nozaki, Tadashige / Tsutsumi, Masahiro / Isumi, Mayu / Shimoda, Shinji / Hamada, Yoshiki / Masutani, Mitsuko
Issue Date: 27-Sep-2019
Publisher: MDPI
Citation: Cells, 8(10), art.no.1157; 2020
Abstract: Poly(ADP-ribose) polymerase (Parp)-1 catalyzes polyADP-ribosylation using NAD+ and is involved in the DNA damage response, genome stability, and transcription. In this study, we demonstrated that aged Parp-1-/- mouse incisors showed more frequent dental dysplasia in both ICR/129Sv mixed background and C57BL/6 strain compared to aged Parp-1+/+ incisors, suggesting that Parp-1 deficiency could be involved in development of dental dysplasia at an advanced age. Computed tomography images confirmed that dental dysplasia was observed at significantly higher incidences in Parp-1-/- mice. The relative calcification levels of Parp-1-/- incisors were higher in both enamel and dentin (p < 0.05). Immunohistochemical analysis revealed (1) Parp-1 positivity in ameloblasts and odontoblasts in Parp-1+/+ incisor, (2) weaker dentin sialoprotein positivity in dentin of Parp-1-/- incisor, and (3) bone sialoprotein positivity in dentin of Parp-1-/- incisor, suggesting ectopic osteogenic formation in dentin of Parp-1-/- incisor. These results indicate that Parp-1 deficiency promotes odontogenic failure in incisors at an advanced age. Parp-1 deficiency did not affect dentinogenesis during the development of mice, suggesting that Parp-1 is not essential in dentinogenesis during development but is possibly involved in the regulation of continuous dentinogenesis in the incisors at an advanced age.
Keywords: poly(ADP-ribose) polymerase-1 / aged mice / knockout mice / dental dysplasia
URI: http://hdl.handle.net/10069/40145
DOI: 10.3390/cells8101157
Rights: © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/40145

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