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Toxoplasma gondiiの生物学的研究 : 第2報 Toxoplasma gondiiの各種理化学的条件に対する抵抗性に関する追加実験


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Title: Toxoplasma gondiiの生物学的研究 : 第2報 Toxoplasma gondiiの各種理化学的条件に対する抵抗性に関する追加実験
Other Titles: Biological Studies on Toxoplasma gondii : II. Further Examination on the Resistance of Toxoplasma gondii to Physico-Chemical Conditions
Authors: 本村, 一郎
Authors (alternative): Motomura, Ichiro
Issue Date: 1967
Publisher: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
Citation: 熱帯医学 Tropical medicine 9(4). p226-243, 1967
Abstract: Following the previous experiment, Toxoplasma gondii was examined in vitro on the resistance to some disinfectants, digestive enzymes and bovine bile. Strains of T. gondii used were the RH and Beverley, and the experimental methods were almost similar as those in the previous experiment. Results obtained were as follows: 1) The parasites and disinfectant which was diluted to different concentrations with saline, were incubated at 4℃ for 15 minutes and washed twice by centrifugation. The parasites were resuspended in saline and inoculated to mice for an examination on infectivity of the parasites. Both forms demonstrated to be able to survive at 4℃ for 15 minutes in 0.1% of saponated cresol, 0.1% benzethonium chloride, 0.01% formalin, 50% ethyl alcohol, 10% sodium bicarbonate and 2% bolic acid. On the other hand, cysts could resist to 5% carbolic acid and 1% thimerosal but proliferative form could do only to 0.1% carbolic acid and 0.001% thimerosal. 2) Resistance to T. gondii to digestive enzymes was examined by incubating them at 37℃ for different hours. It was shown that cysts survived for 5 hours respectively in 1% trypsin, 1% pepsin, 5% diastase and 5% lipase. While, proliferative forms did for 5 hours in 0.5% trypsin and 1% pepsin, and for 3 hours in 5% diastase and 5% lipase. 3) Cysts could survive for 5 hours in 50% bovine bile and for 3 hours in 5% sodium-cholate, but proliferative forms could do for 5 hours in 25% bovine bile and was killed by 0.1% sodium cholate within 30 minutes.
URI: http://hdl.handle.net/10069/4023
ISSN: 03855643
Type: Departmental Bulletin Paper
Appears in Collections:Volume 9, No. 4

Citable URI : http://hdl.handle.net/10069/4023

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