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ECHOウイルス11型の性状に関する研究 : II. Large plaque(Lp)及びSmall plaque(Sp)変異ウイルスとHeLa細胞の系における持続感染について(ECHOウイルス11型の性状に関する研究)


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Title: ECHOウイルス11型の性状に関する研究 : II. Large plaque(Lp)及びSmall plaque(Sp)変異ウイルスとHeLa細胞の系における持続感染について(ECHOウイルス11型の性状に関する研究)
Other Titles: Studies on Mutants of ECHO Virus Type 11 : II. Properties of Carrier Culture of HeLa Cells Infected with Large (Lp) and Small (Sp) Plaque Variants of ECHO Virus Type 11
Authors: 陳, 境津
Authors (alternative): Chen, Jeng Jieng
Issue Date: 30-Jun-1971
Publisher: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
Citation: 熱帯医学 Tropical medicine 13(2). p53-60, 1971
Abstract: Carrier culture of HeLa cells persistently infected with ECHO virus type 11, Gregory strain, produced large plaque (Lp) and small plaque (Sp) variant viruses at the initial period of carrier state. After several passages of subculture, however, Lp virus was eliminated from carrier state and only Sp virus was released in the culture fluid. Such phenomenon was also investigated in case of Sp virus carrier culture challenged with Lp virus. On the other hand, Lp virus carrier culture challenged with Sp virus produced only Sp virus. In order to maintain an equilibrium of such carrier culture, antibody need not be supplied in culture medium. Generation time of Sp virus carrier HeLa cells was given 38.6 hours as same as that of normal HeLa cells. The proportion of infected cells in Sp virus carrier culture was 88.8% at the 11th passage and it reduced to 51.9% at the 25th passage. The subcultures of HeLa cells persistently infected with Sp virus became not produced the virus after the 102nd passage (HeLa sp 102). The adsorption of Lp or Sp virus onto that cells was allowed at the rate of 35% or 85% respectively, and the cells escaped from cytopathic effect caused by virus infection. Culture fluid of HeLa sp 102 cells was adjusted pH to 2.0 by adding N-HCl solution, then, kept at 4℃ for 4 days. After readjusting pH to 7.4, the fluid was centrifuged at 100,000g for 2 hours and the supernatant was used for the test of interferon-like activity. The yield of Sp virus particularly Lp virus in normal HeLa cells treated with the supernatant was markedly reduced. On the other hand, these events were not investigated by heating the supernatant at 60℃ for 1 hour. No effect of the supernatant prepared from normal HeLa cells on the yield of Lp or Sp viruses was observed. Consequently, it would be said that the substance prepared from culture fluid of HeLa sp 102 cells indicated the interferon-like activity and Lp virus was more sensitive to it than Sp virus.
URI: http://hdl.handle.net/10069/4090
ISSN: 03855643
Type: Departmental Bulletin Paper
Appears in Collections:Volume 13, No. 2

Citable URI : http://hdl.handle.net/10069/4090

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