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Title: 日本脳炎ウイルスの形態学の研究
Other Titles: Morphological Studies on Japanese Encephalitis Virus
Authors: 岡, 六四
Authors (alternative): Oka, Rokushi
Issue Date: 30-Sep-1971
Publisher: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
Citation: 熱帯医学 Tropical medicine 13(3). p102-115, 1971
Abstract: Partially purified Japanese encephalitis (JE) virus extracted from infected mouse brains with Diflon 113, a fluorocarbon, seems to be more disrupted than that treated with protamin sulfate. However, the activities for hemagglutination (HA) and complement fixation (CF) antigens of JE virus extracted with Diflon 113 were more maintained than those extracted with aceton-ether. Furthermore, from Diflon extract antigens, HA and CF components were seperated successfully by density gradient centrifugation in CsCl solution containing 0.001M EDTA. The activities for only HA and CF were observed in fractions of ρ=1.310-1.290 and ρ=1.190-1.160, respectively. On the other hand, electron microscopic observations indicated that JE virus particles were about 40-45mμ in diameter and composed of envelope and nucleocapsid. Using the block test, the relation of these components against neutralization antibody was examined. The block test is a examination procedure for remaining antibody in the neutralization system containing antiserum and virus specimen by counting plaque formation of seed virus supplemented. Less or no antibody was blocked with heated viruses at 50℃ or 60℃ for 10 minutes, respectively, and the antibody was blocked with the fraction of ρ=1.130-1.290 but not with the fraction of ρ=1.190-1.160. It was suggested that the block antigen will be heat-labile component, the envelope.
URI: http://hdl.handle.net/10069/4095
ISSN: 03855643
Type: Departmental Bulletin Paper
Appears in Collections:Volume 13, No. 3

Citable URI : http://hdl.handle.net/10069/4095

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