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NAOSITE : Nagasaki University's Academic Output SITE > Institute of Tropical Medicine > Bulletin > Tropical medicine > Volume 14, No. 1 >

緑膿菌のピオシン型別に関する研究 : III. Darrell-Wahba法適用による諸経験

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Title: 緑膿菌のピオシン型別に関する研究 : III. Darrell-Wahba法適用による諸経験
Other Titles: Studies on Pyocine Typing of Pseudomonas aeruginosa : III. Experiences of Darrell-Wahba method, especially on reproducibility of typing results
Authors: 内藤, 達郎 / 岩永, 祥子 / 小浦, 正昭
Authors (alternative): Naito, Tatsuro / Iwanaga, Yoshiko / Koura, Masaaki
Issue Date: 20-Mar-1972
Publisher: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
Citation: 熱帯医学 Tropical medicine 14(1). p1-9, 1972
Abstract: Pyocine typing was carried out by the method of Darrell-Wahba using the total of 165 strains consisting of the same 143 isolates used in the experiment of the first report (J. Jap. Assoc. Inf. Dis., Vo1.45, No.10), of 12 indicators (W1-W12) for this method and of 8 indicators (G1-G8) and 2 type strains (Ty. 1 and Ty. 16) for Gillies-Govan method. From these strains the "la" colonies were isolated, and slant cultures of them were used for typing. Results of the first typing: 15 strains of Type B2, 8 of non-producer, 4 of Type L, 2 of Type B1, one each of Types B3, B4, K and P, and 132 of unclassifiable strains. Among 132 unclassifiable strains, 44 patterns were represented by 1 strain each, 10 patterns by 2 strains, 3 patterns by 3 strains each, and the remaining 7 patterns were observed in the group of 12, 12, 11, 10, 6, 4 and 4 strains respectively. Next, the second typing and the cross experiments were done using 70 strains classified in the definite inhibition pattern or in the group of strains representing two or three patterns appearing similar. Different results obtained from the typing are shown in Tables 1, 3, 4, 5, 7, 8 and 10, and the results of cross reactions in each group are summarized in Tables 2, 6, 9 and 11. About 5 months later, the third typing of the 70 strains mentioned above and the second typing using the remaining 95 strains were performed in order to detect reproducibility of the results. These results as for the third typing are shown in Tables 1, 3, 4, 5, 7, 8 and 10, and those as for the second typing are shown in Tables 12 and 13. The results are summarized as follows: 1. Forty-five out of 70 strains were judged to be reproducible through three times of typing; fourteen strains were typed as B2, but 8 patterns were observed in the group of 11, 6, 4, 3, 2, 2, 2 and 1 strains respectively. 2. In contrast with the results above, only 19 strains were observed as "reproducible" among 95 strains retyped 5 months later. Four strains among them showed inhibition against W10 alone, while 3 were non-producers, and other 12 strains showed 11 different patterns which were not to be found in the typing schema. 3. Applying "Mayo types" of Zabransky and Day, 53 out of 70 strains were found typable and reproducible; 18 strains were typed as B, 16 as D, 13 as F, and the remaining 3, 2 and 1 strains showed each 3 kinds of patterns being different from "Mayo types". Among 95 strains typed twice, 20 strains were found to be reproducible. They were consisted of Type D, represented by 3 strains, of Type B and Type P represented by 1 strain each, of 7 strains of non-producer and of 8 strains representing 6 different patterns respectively as far as the indicators W1-W8 were used. 4. Different inhibition was observed in indicators W5 and W7. From this point of view the present authers can not agree with Zabransky and Day who have omitted W7 in the Mayo typing method. 5. In the cross experiments, most strains in each groups which had been selected by the first typing did not show the cross reactions To discuss "cross immune" within any pyocine type or between types showing similarity in inhibition patterns, further careful investigations are necessary.
URI: http://hdl.handle.net/10069/4106
ISSN: 03855643
Type: Departmental Bulletin Paper
Appears in Collections:Volume 14, No. 1

Citable URI : http://hdl.handle.net/10069/4106

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