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On the Cryo-biological Study of the Parasitic Protozoa : (2) The low temperature preservation in freezers


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Title: On the Cryo-biological Study of the Parasitic Protozoa : (2) The low temperature preservation in freezers
Other Titles: 寄生性原虫類の低温生物学的研究 : 2.フリーザーによる低温保存
Authors: Miyata, Akira
Authors (alternative): 宮田, 彬
Issue Date: 30-Dec-1973
Publisher: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
Citation: 熱帯医学 Tropical medicine 15(4). p204-213, 1973
Abstract: The following results were obtained: 1. Entamoeba histolytica survived for only several days in a sample with 5% dimethyl sulfoxide at -75C after pre-cooling in a -25C freezer. 2. In a sample with 7.5% glycerol stored at -20C, RH strain of Toxoplasma gondii survived for at least 75 days, and Plasmodium berghei was preserved for 49 days, whereas Trypanosoma gambiense survived for only 3 days. 3. T. gambiense, P. berghei, and RH strain of T. gondii could be easily stored at -75C in the presence of 7.5% glycerol, and even without any cryo-protective substance, the latter two species survived for about 80 days. 4. In spite of several trials, Beverley strain of T. gondii could not survive at low temperatures except only one sample stored at -20C for one day had an infectivity to a mouse. 5. The most interesting finding was that the survival period of protozoa in the sample prepared with inactivated bovine serum was somewhat longer than that prepared with physiological saline. / 本報では,先に報告(宮田,1973)したトリコモナス類以外の寄生性原虫類について,-20度,-25度,-75度の3種のフリーザーを用いて,凍結保存を試みた.得られた成績は,次の通りである.1.赤痢アメーバは,凍結保存が困難で,-25度フリーザー中で1時間半予備凍結後,-75度フリーザーヘ移し保存しても,せいぜい数日間保存できたにすぎない.2.7.5%グリセリンを加えた試料を,-20度のフリーザー中に凍結保存する場合は,トキソプラズマRH株は,少なくとも75日,ネズミ・マラリアは,49日保存できたが,ガンビエ・トリパノゾーマは,3日間しか保存できなかった.3.上記3種の原虫は,7.5%グリセリンを加え,-75度フリーザー中で凍結した場合は,大変長期間保存することができる.またトリパノゾーマを除く2種の原虫は,例え凍害保護剤を加えなくても,およそ80日間保存できた.4.何回も試みたが,トキソプラズマのビバリー株の保存は難しく,-20度で凍結した7.5%グリセリン加試料中の1本だけが1日保存後もマウスに感染力をもっていた.しかしその他の試みは全て失敗に終った.5.原虫を非働化牛血清中で凍結する場合は,生理的食塩水中で凍結するよりも,原虫の保存期間が長くなつた.
URI: http://hdl.handle.net/10069/4150
ISSN: 03855643
Type: Departmental Bulletin Paper
Appears in Collections:Volume 15, No. 4

Citable URI : http://hdl.handle.net/10069/4150

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