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Shigella sonnei 100052の産生するバクテリオシンの性状


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Title: Shigella sonnei 100052の産生するバクテリオシンの性状
Other Titles: Properties of Bacteriocin Produced from Shigella sonnei 100052
Authors: 熊沢, 教真 / 内藤, 達郎
Authors (alternative): Kumazawa, Norichika / Naito, Tatsuro
Issue Date: 25-Mar-1975
Publisher: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
Citation: 熱帯医学 Tropical medicine 17(1). p1-11, 1975
Abstract: Shigella sonnei 100052の産生するバクテリオシンの活性を検討した.用いたS. sonneiのほとんどすべての菌株がこのバクテリオシソに感受性であった.限外濾過膜による分画実験の結果,バクテリオシンの分子量は不均一であるがその活性スベクトルは同一であることが明らかになった.バクテリオシンの最小単位の分子量は約28,000であった.バクテリオシンに対するS. sonnei strain 17の感受性はS. sonnei strain 56の感受性よりも高かった.strain 17から分離したバクテリオシン耐性株はバクテリオシンの影響を受けずに増殖した.バクテリオシン産生株S. sonnei 100052はバクテリオシンに感受性であったが,この菌株に対する殺菌作用の発現に要する時間は他の菌株に比較して長時間を要した.S. dysentheriae strain E7はS. sonneiのどの菌株よりもはるかに感受性が高かった.バクテリオシンはKCN, FeSO_4, CuSO_4に感受性,プロナーゼに耐性であった.バクテリオシンの活性はシステインまたはリゾチームによって増強された.strain E7はS. sonneiのコリシン型別法の確立に有用であろうと考えられる. / A bacteriocin produced from Shigella sonnei 100052 was studied kinetically on its activities. Almost all S, sonnei strains seemed to be sensitive to this bacteriocin, Ultrafiltration studies revealed, however, that the bacteriocin had a variety of molecular sizes, whereas their activities seemed to be remain essentially the same. The molecular weight of a minimal unit (monomer) was estimated to be approximately 28,000. The difference in bacteriocin sensitivity among S. sonnei strains were revealed by means of kinetic studies; S. sonnei strain 17 was more sensitive than S. sonnei strain 56, and a resistant variant derived from S. sonnei strain 17 grew whether the bacteriocin was present or not. The survivor curve of S. sonnei type 7 strain, the producer of the bacteriocin, proved that though the strain was sensitive to the bacteriocin, the killing action was delayed. S. dysenteriae strain E7 was far more sensitive to the bacteriocin than S. sonnei strains. The bacteriocin was sensitive to KCN, FeSO_4 and CuSO_4, and resistant to pronase. Its activity was enhanced in the presence of cystein or lysozyme. Strain E7 seemed to be useful to ensure colicin typing of S. sonnei.
URI: http://hdl.handle.net/10069/4174
ISSN: 03855643
Type: Departmental Bulletin Paper
Appears in Collections:Volume 17, No. 1

Citable URI : http://hdl.handle.net/10069/4174

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