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Title: ビブリオシン現象観察用培地の基礎的研究
Other Titles: Basic study on the solid media for the observation of vibriocin activity
Authors: 山口, 恵三
Authors (alternative): Yamaguchi, Keizo
Issue Date: 31-Jul-1976
Publisher: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
Citation: 熱帯医学 Tropical medicine 18(2). p75-90, 1976
Abstract: In the most part of present study, it was necessary to set a fundamental policy that only the parallel experiment on the same day could be compared one another, because of the insufficient reproducibility of the vibriocin phenomenon. The chequer-board experiment in using six strains of classical and El-Tor type Vibrio cholerae respectively was applied to all the experiments excepting the last one. Firstly, it was observed that some strains showed the phenomenon on Anaerobe agar, but not on Tryptosoy agar (Table 1). Accordingly, the result obtained by the experiment in adding 0.05% Na-thioglycollate or glucose to Tryptosoy agar and by another experiment in adding two substances above instantanously were compared (Tables 2-5), then better production was obtained by instantaneous addition, and non-specific inhibition was observed by the use of more than 0.25% of glucose. Further experiments (Tables 5-6) showed that, in preparing the media, non-specific inhibition was eliminated by the use of 0.05M phosphate buffer in place of distilled water. Secondly, applying polypepton agar to the basal medium (Tables 7-10), it was found that the best result was obtained in the medium indicated by the remarks of Table 11. While comparing the incubative temperature and period in the course of production on this medium, better results were observed at 30℃ than 37℃, and much enough vibriocin was produced within 18 hours at both temperatures. Finally, the vibriocins produced by 60 strains of classical and El-Tor type of V. cholerae respectively on the same medium at 30℃ for 18 hours were tested three times for the inhibition to the 12 indicators just the same as mentioned above, As shown in Table 15, indicators 4, 56 and 93 were less sensitive to the vibriocins and indicators 5', 16, 21 and 48 frequently showed doubtful inhibition. Judging from the inhibition test to the remaining five indicators, it was revealed that four strains were unclassifiable as shown in Table 16, and 116 others were classified into 11 patterns as shown in Table 17. These results suggest that the further study on the present subject will make it possible to set up the vibriocin typing method which has no relevancy with the present serotype or biotype.
URI: http://hdl.handle.net/10069/4202
ISSN: 03855643
Type: Departmental Bulletin Paper
Appears in Collections:Volume 18, No. 2

Citable URI : http://hdl.handle.net/10069/4202

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