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緑膿菌のピオシン型別に関する研究 : VI.ピオシン型別法2種と血清型別の併試


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Title: 緑膿菌のピオシン型別に関する研究 : VI.ピオシン型別法2種と血清型別の併試
Other Titles: Studies on Pyocine Typing of Pseudomonas aeruginosa : VI. Parallel application of two pyocine typing methods and serological typing
Authors: 内藤, 達郎 / 小浦, 正昭 / 岩永, 祥子
Authors (alternative): Naito, Tatsuro / Koura, Masaaki / Iwanaga, Yoshiko
Issue Date: 30-Jun-1980
Publisher: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
Citation: 熱帯医学 Tropical medicine 22(2). p101-109, 1980
Abstract: The pyocine typing methods of both Darrell-Wahba and Gillies-Govan, and serological typing of Homma were applied in parallel to each of a la colony from 142 strains which had been used in former reports. The results of pyocine typing obtained were shown in Tables 1-6. Including the typing results of Report V , 54 out of 142 strains which were used in the second experiment of the last report have been typed three times, and there have been two sets of results for remaining 88 strains. Therefore pyocine types of the test strains were determined as a rule in agreement of at least two results in both methods. In Gillies-Govan method, 13 and 27 out of 54 strains showed three and two reproducible patterns respectively in three results, and 68 out of 88 strains were reproducible in two results. Pyocine types determined by reproducible patterns and serological types were summerized in Table 7. On the other hand, 11, 17 and 25 strains respectively showed the same degree of reproducibility as above in Darrell-Wahba method. Applying Yamaguchi's modified scheme (Vol.19 p.42 in this Journal), they were improved to 17, 19 and 39 strains, and resulted pyocine types and serological types were summerized in Table 8. From these results it can be concluded as follows : 1. To determine pyocine type it is necessary to repeat the typing twice or more. 2. Gillies-Govan method is more reproducible than Darrell-Wahba method. 3. There is the relation between serological types and pyocine types to a certain degree, while pyocine types are usefull for subtyping some of serological types. 4. The same kind of relation also exists between two pyocine types (Table 9).
URI: http://hdl.handle.net/10069/4279
ISSN: 03855643
Type: Departmental Bulletin Paper
Appears in Collections:Volume 22, No. 2

Citable URI : http://hdl.handle.net/10069/4279

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