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狂犬病ウイルス免疫脾細胞の抗ウイルス作用


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Title: 狂犬病ウイルス免疫脾細胞の抗ウイルス作用
Other Titles: In vitro Antiviral Activity of Spleen Cells from Mice Immunized with Rabies Virus.
Authors: 牧野, 芳大
Authors (alternative): Making, Yoshihiro
Issue Date: 27-Dec-1980
Publisher: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
Citation: 熱帯医学 Tropical medicine 22(4). p209-221, 1980
Abstract: The effect of mouse spleen cells immunized with either live or beta-propiolactone (BPL)-inactivated rabies virus on the spread of rabies virus was studied in vitro. Spleen cells from C3H/He mice immunized with approximately 5×10^7 focus forming unit of either live or BPL-inactivated rabies virus (Flury-HEP strain) first showed a low level of specific cell-mediated cytotoxicity on day 4 after immunization. The cytotoxicity reached a peak on day 7 and then declined to a low level on day 14. In conjunction with the development of this cytotoxic activity, immune spleen cells significantly suppressed the virus spread in the virus-infected mouse neuroblastoma cells (strain N-18) as revealed by the inhibition of virus specific fluorescent focussize during a 30hr of incubation period. This activity was apparently due to the T lymphocyte populations of immune spleen cells since the activity was not diminished by the depletion of plastic plate adherent cells from spleen cells nor by passage of non-adherent spleen cells through nylon wool column. However, it was abrogated by prior incubation of non-adherent spleen cells with anti-Thy-1 serum and complement. In addition, neither antiviral neutralizing antibody, interferon nor lymphotoxin was detected in the infected murine neuroblastoma cell culture fluid cocultivated with immune spleen cells. This activity was observed only when immune spleen cells and virus infected cells shared at least one H-2 haplotype. This focus size reduction activity was also observed in the spleen cells of mice immunized with BPL-inactivated virus to the same degree as with live virus.
URI: http://hdl.handle.net/10069/4288
ISSN: 03855643
Type: Departmental Bulletin Paper
Appears in Collections:Volume 22, No. 4

Citable URI : http://hdl.handle.net/10069/4288

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