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Brugia pahangiのマウスへの感染実験

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Title: Brugia pahangiのマウスへの感染実験
Other Titles: Experimental Transmission of Brugia pahangi into mice
Authors: 坂本, 信 / 青木, 克己 / 中島, 康雄
Authors (alternative): Sakamoto, Makoto / Aoki, Yoshiki / Nakajima, Yasuo
Issue Date: 31-Mar-1982
Publisher: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
Citation: 熱帯医学 Tropical medicine 24(1). p17-25, 1982
Abstract: The development of Brugia pahangi in ICR strain of mice was studied. Seventy to 200 infective larvae were inoculated subcutaneously in the inguinal region of 63 mice. One to 8 mice were killed at intervals up to 200 days postinoculation. Early developmental stages of larvae were obtained from all mice necropsied between 1 and 13 days postinoculation. Percentage recovery rates ranged from 3 to 40% of the original inoculum. After 20 days, number of animals which yielded live larvae at necropsy decreased progressively with time. Larvae persisted in 5 of 13 animals necropsied 45 and 60 days postinoculation. The recovery rates of larvae from infected mice examined between 15 and 75 days ranged from 0.5 to 36%. The longest period of larval survival was 75 days. Each of 55 mice received 100 infective larvae intraperitoneally. Active larvae were obtained from all mice examined between 3 and 20 days postinoculation. Larvae were detected in 4 of 8 mice necropsied 30 and 45 days postinoculation. The recovery of larvae from infected mice necropsied between 3 and 45 days ranged from 2 to 90%. Live larvae were found in only 4 of 17 mice necropsied between 60 and 150 days postinoculation, and worm recovery rates dropped to only 1%. The life span of B. pahangi in the peritoneal cavity of mice was 150 days at its longest. The location of B. pahangi larvae in mice varied with the site of inoculation. Intraperitoneal inoculation caused all developmental stages of worm to be almost exclusively localized in the peritoneal cavity. Subcutaneous inoculation into the inguinal region produced the different distribution pattern. Larvae were frequently found in the pelt, carcass and fatty tissue in the inguinal and perirenal regions, and some were located in the heart and lungs. The third molt occurred from 7 to 11 days postinoculation. Males started the 4th molt 30 days and females 45 days postinoculation. Sixty days old males gave the appearance of mature adult characterized with the adanal papillae, spicules, gubernaculum, etc. Half of the female worms recovered 60 and 75 days postinoculation, remained in the 4th stage larvae. The remaining females had the morphological characteristics of matured adult female, although microfilariae were not detected in the uterus. The microfilariae were also not detected in the orbital blood and the saline rinsed the peritoneal cavity of the mice infected longer than 60 days; The "patent infection" did not occur in ICR mice.
URI: http://hdl.handle.net/10069/4318
ISSN: 03855643
Type: Departmental Bulletin Paper
Appears in Collections:Volume 24, No. 1

Citable URI : http://hdl.handle.net/10069/4318

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