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トキソプラズマIgG抗体価測定における酵素抗体法(Toxo ELISA Test Kit)に関する検討


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Title: トキソプラズマIgG抗体価測定における酵素抗体法(Toxo ELISA Test Kit)に関する検討
Other Titles: Evaluation of the Enzyme-linked Immunosorbent Assay (Toxo ELISA Test Kit) for the Diagnosis of Toxoplasmosis
Authors: 鈴木, 寛 / 土橋, 賢治 / 宮崎, 昭行 / 中島, ひとみ / 松本, 慶蔵
Authors (alternative): Suzuki, Hiroshi / Tsuchihashi, Kenji / Miyazaki, Teruyuki / Nakashima, Hitomi / Matsumoto, Keizo
Issue Date: 30-Jun-1983
Publisher: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
Citation: 熱帯医学 Tropical medicine 25(2). p91-97, 1983
Abstract: A commercial ELISA-KIT newly developed for the diagnosis or Toxoplasmosis was evaluated by comparing with Dye test and Indirect hemaglutination test (IHA test). A qualitative agreement rate of 93% (65/70) was observed between Dye test and ELISA. But only 76% (53/70) was observed between Dye test and IHA test. So Sensitivity and Speciality of ELISA were almost equal to Dye test, but superior to IHA test especialy in low antibody titer. ELISA value showed plateau in high antibody titer. So we had to dilute the serum of which titer was more than 1280× of IHA test in order to measure the transition of Toxoplasma antibody titer exactly. And that the tecnic of ELISA was very complicated. In general clinical examination IHA test was more convenient than ELISA. So we use ELISA only in special case. And usualy use for another purpose to measure specific IgM antibody which apearance in acute Toxoplasmosis.
URI: http://hdl.handle.net/10069/4352
ISSN: 03855643
Type: Departmental Bulletin Paper
Appears in Collections:Volume 25, No. 2

Citable URI : http://hdl.handle.net/10069/4352

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