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免疫酵素測定法(ELISA)を用いたケニアの人血清のチクングンヤウイルスに対する抗体価の測定


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Title: 免疫酵素測定法(ELISA)を用いたケニアの人血清のチクングンヤウイルスに対する抗体価の測定
Other Titles: Enzyme-linked Immunosorbent Assay (ELISA) for Antibody Titers against Chikungunya Virus on Human Sera from Kenya
Authors: NAKITARE, George W. / 分藤, 桂子 / 五十嵐, 章
Authors (alternative): Nakitare, George W. / Bundo, Keiko / Igarashi, Akira
Issue Date: 30-Sep-1983
Publisher: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
Citation: 熱帯医学 Tropical medicine 25(3). p119-128, 1983
Abstract: ケニア共和国のキスム,ナクール,ナイロビ,ニエリで採血した人血清337検体について,微量間接ELISA法によりチクングンヤ(CHIK)ウイルスに対する抗体価を測定し,抗CHIK-免疫グロブリン(Ig)とIgM抗体の比較を採血地点及び成人と子供について行なった.すべての採血地点において成人は子供に比べてIg-ELISA価の幾何平均値及び抗体保有率共に高かった. CHIKに対する血球凝集抑制又は中和抗体を保有していないがCHIKに対し有意なIg-ELISA抗体価を示す検体が多数存在した.この結果は,採血地点においてCHIKと交叉反応を示す別のアルファウイルスが存在することを示唆すると思われ,このことはマウスの免疫血清を用いた交叉反応によっても推察される. / Antibody titers against Chikungunya (CHIK) virus were determined by indirect ELISA on 337 human sera obtained from Kisumu, Nakuru, Nairobi, and Nyeri areas in the Republic of Kenya. Comparison of anti-CHIK immunoglobulin (Ig-) and IgMantibody occurrence was made on the basis of sampling areas and between adults and children. Adults had higher titers and antibody positive rates of Ig-ELISA than children in all areas sampled. On the other hand, IgM-ELISA levels were low in both children and adults. Many specimens with significant levels of Ig-ELISA did not possess hemag-glutination inhibition or neutralization antibodies against CHIK. This result appears to suggest the occurrence of some alphavirus(es) in the study areas cross-reacting with CHIK, as suggested by the cross-ELISA tests using mouse immune sera.
URI: http://hdl.handle.net/10069/4356
ISSN: 03855643
Type: Departmental Bulletin Paper
Appears in Collections:Volume 25, No. 3

Citable URI : http://hdl.handle.net/10069/4356

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