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Title: 単球及びリンパ球由来白血球性発熱物質によるウサギ発熱に関する研究
Other Titles: Studies on Pyrogenic Fever Induced by Granulocytes-Free Leukocytes in Rabbits
Authors: 藤原, 真理子 / 岩元, 純 / 大渡, 伸 / 土屋, 勝彦 / 小坂, 光男 / 柳, 忠道 / 辻, 芳郎 / Hw, Soeliadi
Authors (alternative): Fujiwara, Mariko / Iwamoto, Jun / Ohwatari, Nobu / Tsuchiya, Katsuhiko / Kosaka, Mitsuo / Yanagi, Tadamichi / Tsuji, Yoshiro / Hw, Soeliadi
Issue Date: 31-Mar-1984
Publisher: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
Citation: 熱帯医学 Tropical medicine 26(1). p37-41, 1984
Abstract: 顆粒球を含まない白血球から生成した発熱物質を用いてウサギの発熱曲線を解析した.ウサギ全血からFicoll-Conray比重遠心法にて単球とリンパ球を分離し,37℃の炭酸ガス培養器中で,大腸菌由来のIipopolysaccharide(LPS)を2時間感作させて,内因性発熱物質(LP)を含む培養液を得た.この培養上清液をウサギに静注したところ,10~15分の短い潜時を経て二峰性の発熱が現われた.この直腸温上昇に伴い,耳介皮膚温の二度にわたる下降が観察された.発熱の持続時間は通常2~3時間以内であった.しかしながら,シクロオキシゲナーゼの合成阻害剤であるアセテルサリチル酸を培養液に添加して得られたLP投与では,一峰目の直腸温のわずかな上昇は,減少あるいは消失し,それに伴い,耳介皮膚温の初期下降相の持続時間も短縮された.以上の結果をもとに,内因性発熱物質の作用について新たな考察を加えた. / The ability of rabbits' mononuclear cells to release a leukocytic pyrogen (LP) in vitro and its pyrogenecity were studied in the rabbits. Crude LP was obtained from granulocytes-free leukocytes as follows; the mixed cells of lymphocytes and monocytes had been separated on Ficoll-Conray gradients from whole blood of rabbits, and these cells were sensitized with bacterial lipopolysaccharide (LPS) in RPMI 1640 solution for two hours at 37℃ in the presence or absence of acetylsalicylate. To estimate the pyrogenecity of LP, these crude pyrogens were intravenously applied to conscious rabbits. A typical response to LP was characterized by a rapid onset and short lasting of fever; after the short delay of 10 to 15 minutes, biphasic fever curve was developed following the two phases of decreased ear skin temperature. When acetylsalicylate, an inhibitor of cyclooxygenase, was added to culture medium before beginning of incubation, the first slight rise of rectal temperature became smaller or disappeared, and the duration of initial fall of ear skin temperature was shortened. Further, underlying mechanism of these crude LP-induced fever was discussed in this paper.
URI: http://hdl.handle.net/10069/4375
ISSN: 03855643
Type: Departmental Bulletin Paper
Appears in Collections:Volume 26, No. 1

Citable URI : http://hdl.handle.net/10069/4375

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