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コレラ菌の生物学的性状特にヒツジ赤血球溶血性の地理・年代的変異


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タイトル: コレラ菌の生物学的性状特にヒツジ赤血球溶血性の地理・年代的変異
その他のタイトル: Geographical and Chronological Changes of Biological Properties Especially Hemolysis to Sheep Erythrocytes among Vibrio cholerae O-1
著者: 一瀬, 休生
著者(別表記) : Ichinose, Yoshio
発行日: 1985年 6月29日
出版者: 長崎大学熱帯医学研究所 / Institute of Tropical Medicine, Nagasaki University
引用: 熱帯医学 Tropical medicine 27(2). p53-66, 1985
抄録: The seventh cholera pandemic originated from Celebes islands in 1961 is still continuing, resulting in the frequent epidemics of cholera in the tropics such as Southeast Asian and African countries. The changes in biological characters of Vibrio cholerae are occuring (Samadi et al., 1983) and multiple drug-resistant Vibrio cholerae has also emerged in Tanzania (Towner et al., 1980) and Bangladesh (Glass et al., 1980). A total of 367 strains of Vibrio cholerae which were isolated in Kenya in 1983 and 1984, and in the Philippines in 1982 and 1984 were characterized and analyzed epidemiologically in this study. The majority of strains examined were eltor, Ogawa type. In prophage typing, three strains from the Philippines isolated in 1982 were Ubol type and one from Kenya isolated in 1983 and six from the Philippines isolated in 1984 were Celebes cured type. The majority was Celebes original type. Drug sensitivity test against chloramphenicol, tetracycline, streptomycin, ampicillin and nalidixic acid revealed that chloramphenicol and nalidixic acid had very high vibriocidal effect, but that 266 strains out of 338 (78.6%) isolated in Kenya were resistant to tetracycline, streptomycin and ampicillin. On the other hand, the strains isolated in the Philippines were sensitive to all drugs except one resistant against streptomycin and ampicillin and five resistant to streptomycin. At the same time, a total of 253 strains out of 317 (79.8%) from Kenya and the Philippines were revealed to be resistant to O/129 (Vibriostatic agent) which has been used to differentiate vibrio groups from other gram negative bacilli. Hemolytic activity of Vibrio cholerae were checked by four kinds of methods; 1) the modified method of Feeley and Pittman recommended by Zinnaka using heart infusion broth (pH 7.4), 2) 1% glycerolized heart infusion broth method (Barua and Mukherjee, 1964), 3) 6 hours incubation method, and 4) the method of Barrett and Blake (1981). Vibrio cholerae isolated in Kenya, consequently, showed higher hemolytic activity than those from the Philippines in the four methods, and the difference was statistically significant (x^2-test, P<0.01). The hemolytic activity was also compared by year. The results showed that, in the Philippines, it was significantly higher in 1982 than in 1984.
URI: http://hdl.handle.net/10069/4403
ISSN: 03855643
資料タイプ: Departmental Bulletin Paper
出現コレクション:第27巻 第2号

引用URI : http://hdl.handle.net/10069/4403

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